Expression of transferrin receptor and ferritin following ferumoxides-protamine sulfate labeling of cells: implications for cellular magnetic resonance imaging
Expression of transferrin receptor and ferritin following ferumoxides-protamine sulfate labeling of cells: implications for cellular magnetic resonance imaging
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DOI：
10.1002/nbm.1038
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发表时间：
2006-08-01
影响因子：
2.9
通讯作者：
Frank, Joseph A.
中科院分区：
文献类型：
--
作者：
Pawelczyk, Edyta;Arbab, Ali S.;Frank, Joseph A.
阿魏氧化铁-硫酸鱼精蛋白（FE-Pro）复合物用于细胞内磁标记，通过体内MRI无创监测细胞运输。FE-Pro标记对细胞无毒；然而，FE-Pro标记对转铁蛋白受体（TfR-1）和铁蛋白（参与铁运输和储存的蛋白质）细胞表达的影响尚未见报道。fe - pro标记的人间充质干细胞（MSCs）、HeLa细胞和原代巨噬细胞培养1周至2个月，RT-PCR检测TfR-1和铁蛋白基因表达，Western blots检测蛋白水平。进行MTT（增殖试验）和活性氧（ROS）分析。FE-Pro标记HeLa和MSCs导致TfR-1 mRNA和蛋白水平瞬间下降。相比之下，Fe-Pro标记原代巨噬细胞导致TfR-1 mRNA升高，但TfR-1蛋白水平未升高。铁蛋白mRNA和蛋白水平在标记的HeLa和巨噬细胞中短暂升高，但在MSCs中持续升高。MTT和ROS分析未见变化。总之，FE-Pro标记引起了铁代谢或储存的生理变化，验证了该方法用于MRI细胞跟踪的安全性。2006年由John Wiley & Sons出版。
Ferumoxides-protamine sulfate (FE-Pro) complexes are used for intracellular magnetic labeling of cells to non-invasively monitor cell trafficking by in vivo MRI. FE-Pro labeling is non-toxic to cells; however, the effects of FE-Pro labeling on cellular expression of transferrin receptor (TfR-1) and ferritin, proteins involved in iron transport and Storage, has not been reported. FE-Pro-labeled human mesenchymal stem cells (MSCs), HeLa cells and primary macrophages were cultured from I week to 2 months and evaluated for TfR-1 and ferritin gene expression by RT-PCR and protein levels were determined using Western blots. MTT (proliferation assay) and reactive oxygen species (ROS) analysis were performed. FE-Pro labeling of HeLa and MSCs resulted in a transient decrease in TfR-1 mRNA and protein levels. In contrast, Fe-Pro labeling of primary macrophages resulted in an increase in TfR-1 mRNA but not in TfR-1 protein levels. Ferritin mRNA and protein levels increased transiently in labeled HeLa and macrophages but were sustained in MSCs. No changes in MTT and ROS analysis were noted. In conclusion, FE-Pro labeling elicited physiological changes of iron metabolism or storage, validating the safety of this procedure for cellular tracking by MRI. Published in 2006 by John Wiley & Sons, Ltd.