Identification and characterization of small RNAs in Yersinia pestis.
Identification and characterization of small RNAs in Yersinia pestis.
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DOI：
10.4161/rna.23590
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发表时间：
2013-03
期刊：
影响因子：
4.1
通讯作者：
Wade JT
中科院分区：
文献类型：
--
作者：
Beauregard A;Smith EA;Petrone BL;Singh N;Karch C;McDonough KA;Wade JT
鼠疫耶尔森氏菌是鼠疫的病原体，在进化上与假结核耶尔森氏菌密切相关，但具有非常不同的感染模式。RNA结合调节蛋白Hfq通过小RNA（sRNA）介导调节，并且是Y.鼠疫菌和Y.假结核此外，Hfq是Y生长所必需的。鼠疫菌，而不是Y.假结核，37°C。总之，这些观察结果表明，sRNA在Y的毒力和生存中起着重要作用。sRNA的调节可能是鼠疫菌与鼠疫杆菌之间差异的原因之一。鼠疫菌和Y.假结核我们使用深度测序方法鉴定了Y中的31种sRNA。鼠疫这些sRNA中的大多数在耶尔森氏菌之外不保守。通过北方分析证实sRNA的表达，并且我们开发了深度测序方法以同时定位许多sRNA的5 ′端和3 ′端。我们鉴定的大多数sRNA的表达取决于HFq。我们还观察到温度对许多sRNA表达的依赖性影响，以及Y.鼠疫菌和Y.假结核因此，我们的数据表明，sRNA的调节在从跳蚤到哺乳动物宿主的生活方式转换中起着重要作用，并且sRNA的调节可能有助于Y.鼠疫菌和Y.假结核
Yersinia pestis, the etiologic agent of plague, is closely related to Yersinia pseudotuberculosis evolutionarily but has a very different mode of infection. The RNA-binding regulatory protein, Hfq, mediates regulation by small RNAs (sRNAs) and is required for virulence of both Y. pestis and Y. pseudotuberculosis. Moreover, Hfq is required for growth of Y. pestis, but not Y. pseudotuberculosis, at 37°C. Together, these observations suggest that sRNAs play important roles in the virulence and survival of Y. pestis, and that regulation by sRNAs may account for some of the differences between Y. pestis and Y. pseudotuberculosis. We have used a deep sequencing approach to identify 31 sRNAs in Y. pestis. The majority of these sRNAs are not conserved outside the Yersiniae. Expression of the sRNAs was confirmed by Northern analysis and we developed deep sequencing approaches to map 5ʹ and 3ʹ ends of many sRNAs simultaneously. Expression of the majority of the sRNAs we identified is dependent upon Hfq. We also observed temperature-dependent effects on the expression of many sRNAs, and differences in expression patterns between Y. pestis and Y. pseudotuberculosis. Thus, our data suggest that regulation by sRNAs plays an important role in the lifestyle switch from flea to mammalian host, and that regulation by sRNAs may contribute to the phenotypic differences between Y. pestis and Y. pseudotuberculosis.