Sequential biomolecular, macrofossil, and microfossil extraction from coprolites for reconstructing past behavior and environments

Sequential biomolecular, macrofossil, and microfossil extraction from coprolites for reconstructing past behavior and environments
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DOI:
10.3389/fevo.2023.1131294
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发表时间:
2023-04
期刊:
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影响因子:
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通讯作者:
John C. Blong;H. Whelton;Eline N. van Asperen;I. Bull;L. Shillito
John C. Blong;H. Whelton;Eline N. van Asperen;I. Bull;L. Shillito
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文献类型:
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作者:
John C. Blong;H. Whelton;Eline N. van Asperen;I. Bull;L. Shillito

文献摘要

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从粪化石中回收的宏观、微观和生物分子遗骸的研究可以提供关于过去人类和动物行为和环境的广泛信息。在早期的研究中,研究往往集中在一个或两个代理,但多代理的方法结合数据从所有仍然在粪化石变得越来越普遍。多重代理分析已证明对加强我们对过去的理解和减少等同性的价值。在这里,我们提出了一个连续的生物分子,宏化石,和微化石提取协议,分离所有不同的粪化石成分,并打算作为粪化石分析的最佳实践指南。我们证明了这种方法的有效性,将其应用到一个组合的粪化石从佩斯利洞穴,俄勒冈州,美国。通过结合广泛的代理,这项研究提供了重要的信息,在过去的生物分类和行为,以及行为的古生态环境。
The study of macroscopic, microscopic, and biomolecular remains recovered from coprolites can provide a wide range of information on past human and animal behavior and environments. In earlier studies, research tended to focus on one or two proxies, but multiproxy approaches combining data from all remains within coprolites are becoming more common. Multiproxy analyses have demonstrated value for strengthening our understanding of the past and reducing equifinality. Here we present a sequential biomolecular, macrofossil, and microfossil extraction protocol that separates all different coprolite components and is intended as a best-practice guideline for coprolite analysis. We demonstrate the effectiveness of this method by applying it to an assemblage of coprolites from the Paisley Caves, Oregon, USA. By combining a wide range of proxies, this study provides important information on the taxonomy and behavior of organisms in the past as well as the paleoecological context of behavior.