CD109 promotes the tumorigenic ability and metastatic motility of pancreatic ductal adenocarcinoma cells
CD109 promotes the tumorigenic ability and metastatic motility of pancreatic ductal adenocarcinoma cells
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DOI：
10.1016/j.pan.2020.01.013
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发表时间：
2020-04-01
期刊：
影响因子：
3.6
通讯作者：
Sugamura, Kazuo
中科院分区：
文献类型：
--
作者：
Hatsuzawa, Yuuri;Yamaguchi, Kazunori;Sugamura, Kazuo
背景：越来越多的证据表明，CD109是一种糖基磷脂酰肌醇锚定的糖蛋白，在包括胰腺在内的多个器官的人上皮癌中高表达，但其在癌症发生中的功能作用尚未完全阐明。本研究旨在探讨CD109在胰腺导管腺癌（PDAC）恶性肿瘤中的作用。方法：对145例PDAC标本进行CD109免疫染色，分析CD109表达与临床病理情况的相关性。通过 siRNA 或 shRNA 降低 PANC-1 细胞（一种 PDAC 衍生细胞系）中的 CD109 表达，并检查其对 PANC-1 细胞恶性肿瘤的影响。结果：抑制 PANC-1 细胞中的 CD109 表达导致体外细胞运动性和异种移植物中的致瘤性降低。基于这些结果，我们利用肿瘤组织标本研究了 CD109 表达与 PDAC 转移之间的关系。在145例PDAC的106例复发病例中，CD109阳性病例有伴有远处转移的倾向。结论：CD109在促进PDAC细胞的致瘤能力和与转移相关的细胞运动方面发挥着关键作用。 (C) 2020 年 IAP 和 EPC。由 Elsevier B.V. 出版。保留所有权利。
Background: Accumulating evidence indicates that CD109, a glycosylphosphatidylinositol-anchored glycoprotein, is highly expressed in human epithelial carcinomas of multiple organs including the pancreas, but its functional role in carcinoma development has not yet been fully clarified. The aim of this study was to investigate the role of CD109 in the malignancy of pancreatic ductal adenocarcinoma (PDAC).Methods: PDAC specimens of 145 cases were immunostained for CD109, and correlations between CD109 expression and clinicopathological conditions were analyzed. CD109 expression in PANC-1 cells, a PDAC-derived cell line, was decreased by siRNA or shRNA and its effect on the malignancy of PANC-1 cells was examined.Results: Suppression of CD109 expression in PANC-1 cells resulted in reduction of in vitro cell motility and tumorigenicity in xenografts. Based on these results, we investigated the relationship between CD109 expression and metastasis of PDAC using tumor tissue specimens. Among 106 recurrent cases of 145 PDAC, there was a tendency for CD109-positive cases to be accompanied by distant metastasis.Conclusions: CD109 plays a critical role in the promotion of tumorigenic ability and cellular motility relating to metastasis of PDAC cells. (C) 2020 IAP and EPC. Published by Elsevier B.V. All rights reserved.