Metallothionein-I promoter-directed expression of foreign proteins in a mouse pituitary corticotrope tumor cell line.
Metallothionein-I promoter-directed expression of foreign proteins in a mouse pituitary corticotrope tumor cell line.
复制标题
金属硫蛋白-I 启动子指导外源蛋白在小鼠垂体促肾上腺皮质激素肿瘤细胞系中的表达。
DOI：
10.1016/0303-7207(89)90147-0
复制
发表时间：
1989
影响因子：
4.1
通讯作者：
Mains,RE
中科院分区：
文献类型：
--
作者：
Dickerson,IM;Peden,KW;Mains,RE
Expressing foreign proteins in heterologous eukaryotic cells has been a powerful tool for analyzing protein structure and function. The inducible mouse metallothionein-I promoter has been particularly useful for expression studies. However, the levels of expression achieved with this promoter in heterologous eukaryotic expression systems have not equaled those observed in vivo for the metallothionein-I gene. We have constructed expression plasmids placing either the gene for chloramphenicol acetyltransferase (CAT) or the cDNA for human neuropeptide Y (NPY) under control of the mouse metallothionein-I promoter. These two expression vectors were used to transfect mouse anterior pituitary tumor cells, from which stable transformants were isolated. The resulting cell lines, Mt.NPY1a and Mt.CAT, were used to maximize functional product expression from the metallothionein-I promoter. In both cell lines, a 35-fold induction of mRNA accumulation, peptide synthesis, or CAT activity was observed.
