Stab wound injury of the zebrafish telencephalon: A model for comparative analysis of reactive gliosis
Stab wound injury of the zebrafish telencephalon: A model for comparative analysis of reactive gliosis
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DOI：
10.1002/glia.22269
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发表时间：
2012-03-01
期刊：
影响因子：
6.2
通讯作者：
Ninkovic, Jovica
中科院分区：
文献类型：
--
作者：
Baumgart, Emily Violette;Barbosa, Joana S.;Ninkovic, Jovica
反应性神经胶质细胞，包括星形胶质细胞和少突胶质细胞祖细胞（OPC）是在哺乳动物大脑中的损伤反应的核心，最初是有益的，后来是部分不利的功能，如瘢痕形成。鉴于不同的胶质细胞组成的成年斑马鱼脑放射状室管膜胶质细胞，但没有实质星形胶质细胞，我们研究了神经胶质细胞的反应，在成年斑马鱼端脑的侵入性刺伤损伤模型。令人惊讶的是，受伤后几天，伤口已经愈合，没有任何疤痕组织。与哺乳动物相似，小胶质细胞首先反应并聚集在损伤部位附近，而GFAP+放射状室管膜胶质细胞和成体OPCs均未被招募到损伤部位。此外，OPCs在损伤后未能增加其增殖，而增殖的GFAP+胶质细胞的数量增加，直到损伤后7天。重要的是，损伤后神经发生也增加，产生额外的神经元招募到实质中存活数月。因此，这些数据表明，在成年斑马鱼端脑的特定的神经胶质环境不仅是长期的神经元存活许可，但避免疤痕形成。因此，成年斑马鱼端脑的侵入性损伤可能提供一个有用的模型来解开这些有益的神经胶质反应的分子机制。(C)2011 Wiley Periodicals，Inc.
Reactive glia, including astroglia and oligodendrocyte progenitors (OPCs) are at the core of the reaction to injury in the mammalian brain with initially beneficial and later partially adverse functions such as scar formation. Given the different glial composition in the adult zebrafish brain with radial ependymoglia but no parenchymal astrocytes, we examined the glial response to an invasive stab wound injury model in the adult zebrafish telencephalon. Strikingly, already a few days after injury the wound was closed without any scar tissue. Similar to mammals, microglia cells reacted first and accumulated close to the injury site, while neither GFAP+ radial ependymoglia nor adult OPCs were recruited to the injury site. Moreover, OPCs failed to increase their proliferation after this injury, while the number of proliferating GFAP+ glia was increased until 7 days after injury. Importantly, neurogenesis was also increased after injury, generating additional neurons recruited to the parenchyma which survived for several months. Thus, these data suggest that the specific glial environment in the adult zebrafish telencephalon is not only permissive for long-term neuronal survival, but avoids scar formation. Invasive injury in the adult zebrafish telencephalon may therefore provide a useful model to untangle the molecular mechanisms involved in these beneficial glial reactions. (C) 2011 Wiley Periodicals, Inc.