Patterning proteins and cells using soft lithography
Patterning proteins and cells using soft lithography
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DOI：
10.1016/s0142-9612(99)00165-9
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发表时间：
1999-12-01
期刊：
影响因子：
14
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Kane, RS;Takayama, S;Whitesides, GM
本综述描述了使用非光刻微加工技术的蛋白质和细胞的图案，我们称之为“软光刻”，因为它由一组相关技术组成，每个技术都使用弹性（“软”）材料制造的印章或通道进行图案转移。本文综述了三种软光刻技术：微接触印刷、使用微流体通道的图案和层流图案。这些软光刻技术价格低廉，程序简单，可用于各种平面和非平面基材的图案。它们的成功应用不需要严格的实验室环境调节，它们可以用精致的配体来图案表面。它们提供了对表面化学和细胞环境的控制。我们讨论了基于这些软光刻技术的图像化过程，以及它们在生物传感器技术、组织工程和细胞生物学基础研究中的应用。1999爱思唯尔科学有限公司版权所有。
This review describes the pattering of proteins and cells using a non-photolithographic microfabrication technology, which we call 'soft lithography' because it consists of a set of related techniques, each of which uses stamps or channels fabricated in an elastomeric ('soft') material for pattern transfer. The review covers three soft lithographic techniques: microcontact printing, patterning using microfluidic channels, and laminar flow patterning. These soft lithographic techniques are inexpensive, are procedurally simple, and can be used to pattern a variety of planar and non-planar substrates. Their successful application does not require stringent regulation of the laboratory environment, and they can be used to pattern surfaces with delicate ligands. They provide control over both the surface chemistry and the cellular environment. We discuss both the procedures for patterning based on these soft lithographic techniques, and their applications in biosensor technology, in tissue engineering, and for fundamental studies in cell biology. (C) 1999 Elsevier Science Ltd. All rights reserved.