Varying specificity of systemic lupus erythematosus sera for histone fractions and a periodate-sensitive antigen associated with histone.
Varying specificity of systemic lupus erythematosus sera for histone fractions and a periodate-sensitive antigen associated with histone.
复制标题
系统性红斑狼疮血清对组蛋白组分和与组蛋白相关的高碘酸盐敏感抗原的不同特异性。
DOI：
10.4049/jimmunol.103.4.804
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发表时间：
1969
影响因子：
4.4
通讯作者：
B. Stollar
中科院分区：
文献类型：
--
作者：
B. Stollar
三种人类系统性红斑狼疮(SLE)血清与游离组蛋白反应，对组蛋白组分显示出不同的选择性。两份血清主要与F1组蛋白反应，一份血清与F2a反应。另一种血清似乎与F2a2组蛋白发生特异性反应，但与该血清反应的抗原被高碘酸盐灭活，并通过电泳法从组蛋白中去除。第五种血清不与游离DNA或游离组蛋白反应，与组蛋白-DNA复合体反应，但仅当它们与F2a2组蛋白形成时才反应；该组蛋白抗原在高碘酸盐作用后保持活性，并在制备电泳上与均一的F2a2蛋白恢复。血清与游离组蛋白和核蛋白的相对反应性也不同。
Three human systemic lupus erythematosus (SLE) sera reacted with free histones and showed varying selectivity for the histone fractions. Two sera were primarily reactive with F1 histone and one with F2a. Another serum appeared to react specifically with F2a2 histone, but the antigen which reacted with this serum was inactivated by periodate and was removed from the histone by electrophoresis. A fifth serum, which did not react with free DNA or free histone, reacted with histone-DNA complexes, but only when they were formed with the F2a2 histone; this component of histone antigen remained active after exposure to periodate and was recovered with homogeneous F2a2 protein on preparative electrophoresis. The sera also varied in their relative reactivities with free histone and nucleoprotein.