Preventing arginine-to-proline conversion in a cell-line-independent manner during cell cultivation under stable isotope labeling by amino acids in cell culture (SILAC) conditions
Preventing arginine-to-proline conversion in a cell-line-independent manner during cell cultivation under stable isotope labeling by amino acids in cell culture (SILAC) conditions
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DOI：
10.1016/j.ab.2011.01.011
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发表时间：
2011-05-01
影响因子：
2.9
通讯作者：
Schnoelzer, Martina
中科院分区：
文献类型：
--
作者：
Loessner, Christopher;Warnken, Uwe;Schnoelzer, Martina
定量蛋白质组学作为一种描述不同细胞状态下蛋白质表达变化的工具，在生命科学研究中的影响越来越大。细胞培养中氨基酸稳定同位素标记（SILAC）是一种有效的蛋白质相对定量技术。然而，精氨酸代谢转化为脯氨酸，导致额外的重标记脯氨酸肽卫星，从而损害了定量的准确性。在这里，我们重新研究了在SILAC条件下细胞培养过程中添加未标记的脯氨酸，考虑了数千种肽，并证明了脯氨酸向脯氨酸的转化被阻止，与所使用的细胞系无关。(C)2011 Elsevier Inc. All rights reserved.
Quantitative proteomics has increasingly gained impact in life science research as a tool to describe changes in protein expression between different cellular states. Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful technique for relative quantification of proteins. However, the accuracy of quantification is impaired by the metabolic conversion of arginine to proline resulting in additional heavy labeled proline peptide satellites. Here we reinvestigated the addition of unlabeled proline during cell cultivation under SILAC conditions considering several thousand peptides and demonstrated that the arginine-to-proline conversion is prevented independent of the cell line used. (C) 2011 Elsevier Inc. All rights reserved.