Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside
Mesenchymal Stem Cell Concentration and Bone Repair: Potential Pitfalls from Bench to Bedside
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DOI：
10.2106/jbjs.h.00303
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发表时间：
2009-05-01
影响因子：
5.3
通讯作者：
Lieberman, Jay R.
中科院分区：
文献类型：
--
作者：
Cuomo, Anna V.;Virk, Mandeep;Lieberman, Jay R.
背景：骨髓间充质干细胞具有多向分化潜能，具有向骨组织分化的能力。我们进行了一项临床前试验，比较成骨潜能的人骨髓穿刺液与间充质干细胞富集的骨髓穿刺液（两者混合脱钙骨基质）在一个临界大小的大鼠femoral defect model.Methods：血沉棕黄层从人骨髓穿刺液中提取，以获得间充质干细胞富集的骨髓穿刺液。将59只无胸腺大鼠（各有6 mm股骨缺损）分为6个处理组：仅缺损（组I），脱矿骨基质和盐水溶液（II组），脱钙骨基质和骨髓穿刺液（III组），脱钙骨基质和富含间充质干细胞的骨髓穿刺液（IV组）、脱钙骨基质和重组人骨形态发生蛋白-2（rhBMP-2）（V组[阳性对照]）和可吸收胶原海绵和rhBMP-2（VI组[阳性对照]）。所有动物在12周被杀的放射照相，显微计算机断层扫描，组织形态学，和组织学analysis.Results：有广泛的变异间充质干细胞的浓度从人类捐助者。阳性对照组（第V组和第VI组）的所有10个缺损均愈合。在每个实验组（组II、III和IV）中仅一个缺损愈合（即，44个缺损中有3个愈合）。第II、III和IV组的放射学评分之间无显著差异（p = 0.59），且各组的评分均显著高于第I组（p
Background: Mesenchymal stem cells are multipotent and have the ability to differentiate into bone. We conducted a preclinical trial comparing the osteogenic potential of human bone marrow aspirate with that of mesenchymal stem cell-enriched bone marrow aspirate (both mixed with demineralized bone matrix) in a critical-sized rat femoral defect model.Methods: The buffy coat was extracted from human bone marrow aspirate to obtain mesenchymal stem cell-enriched bone marrow aspirate. Fifty-nine athymic rats, each with a 6-mm femoral defect, were divided into six treatment groups: defect only (Group I), demineralized bone matrix and saline solution (Group II), demineralized bone matrix and bone marrow aspirate (Group III), demineralized bone matrix and mesenchymal stem cell-enriched bone marrow aspirate (Group IV), demineralized bone matrix and recombinant human bone morphogenetic protein-2 (rhBMP-2) (Group V [positive control]), and absorbable collagen sponge and rhBMP-2 (Group VI [positive control]). All animals were killed at twelve weeks for radiographic, micro-computed tomography, histomorphometric, and histologic analysis.Results: There was wide variability in the mesenchymal stem cell concentrations obtained from the human donors. All ten defects healed in the positive control groups (Groups V and VI). Only one defect healed in each experimental group (Groups II, III, and IV) (i.e., three of forty-four defects healed). There was no significant difference among the radiographic scores of Groups II, III, and IV (p = 0.59), and the score for each of those groups was significantly higher than that for Group I (p