Activation of the meiotic divisions in Drosophila oocytes
Activation of the meiotic divisions in Drosophila oocytes
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DOI：
10.1006/dbio.1997.8506
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发表时间：
1997-03-15
影响因子：
2.7
通讯作者：
OrrWeaver, TL
中科院分区：
文献类型：
--
作者：
Page, AW;OrrWeaver, TL
在许多生物体中，关键的减数分裂事件是在翻译水平上控制的。在这项研究中，我们研究了翻译调控在果蝇减数分裂细胞周期中的作用。为了解决这个问题，我们开发了一种在体外激活果蝇卵母细胞的系统。通过这种方法，可以激活数百个成熟卵母细胞恢复并完成减数分裂。根据细胞学标准，减数分裂的阶段是正常的，减数分裂的时间与体内激活的卵子相似。我们使用这个系统来研究蛋白质合成在调节减数分裂进程和维持中期I停滞中的作用。我们发现中期I后新蛋白的合成不需要后期I、减数分裂II或减数分裂产物的去浓缩。此外，维持中期I阻滞不需要持续的蛋白质合成。然而，减数分裂后染色质的重新凝聚需要新的蛋白质合成。(C) 1997学术出版社。
Key meiotic events in many organisms are controlled at the translational level. In this study, we examine the role of translational regulation in the meiotic cell cycle of Drosophila. In order to address this question, we developed a system for activating Drosophila oocytes in vitro. With this method, hundreds of mature oocytes can be activated to resume and complete meiosis. The stages of meiosis are normal by cytological criteria, and the timing of the meiotic divisions is similar to that of eggs activated in vivo. We use this system to examine the role of protein synthesis in regulating the progression of meiosis and the maintenance of the metaphase I arrest. We find that synthesis of new proteins after metaphase I is not required for anaphase I, meiosis II, or the decondensation of the meiotic products. Also, continued protein synthesis is not required to maintain the metaphase I arrest. New protein synthesis is required, however, for proper chromatin recondensation after meiosis. (C) 1997 Academic Press.