Localization microscopy coming of age: from concepts to biological impact
Localization microscopy coming of age: from concepts to biological impact
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DOI：
10.1242/jcs.123612
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发表时间：
2013-08-15
影响因子：
4
通讯作者：
Sauer, Markus
中科院分区：
文献类型：
--
作者：
Sauer, Markus
通过单分子光活化或光开关和位置确定（定位显微镜）的超分辨率荧光成像有可能从根本上彻底改变我们对细胞功能如何在分子水平上编码的理解。在近年来推出的所有强大的高分辨率成像技术中，定位显微镜表现出色，因为它提供了有关分子分布的单分子信息，甚至给出了亚细胞区室中存在的蛋白质的绝对数量。这提供了在分子水平上对生物系统的深入了解，可以产生直接的实验反馈，用于模拟生物相互作用的复杂性。此外，有效的新的标记方法和策略，以提高本地化正在出现，有望实现真正的分子分辨率。这提高了本地化显微镜相关的光学和电子显微镜实验作为一个强大的补充方法。
Super-resolution fluorescence imaging by single-molecule photoactivation or photoswitching and position determination (localization microscopy) has the potential to fundamentally revolutionize our understanding of how cellular function is encoded at the molecular level. Among all powerful, high-resolution imaging techniques introduced in recent years, localization microscopy excels because it delivers single-molecule information about molecular distributions, even giving absolute numbers of proteins present in subcellular compartments. This provides insight into biological systems at a molecular level that can yield direct experimental feedback for modeling the complexity of biological interactions. In addition, efficient new labeling methods and strategies to improve localization are emerging that promise to achieve true molecular resolution. This raises localization microscopy as a powerful complementary method for correlative light and electron microscopy experiments.