Complete murine cDNA sequence, genomic structure, and tissue expression of the high mobility group protein HMG-I(Y).
Complete murine cDNA sequence, genomic structure, and tissue expression of the high mobility group protein HMG-I(Y).
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高迁移率族蛋白 HMG-I(Y) 的完整鼠 cDNA 序列、基因组结构和组织表达。
DOI：
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发表时间：
1988
影响因子：
4.8
通讯作者：
Raymond Reeves
中科院分区：
文献类型：
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作者：
Kenneth R. Johnson;Donald A. Lehnt;Terry;Eltonll;Philip;Barr;Raymond Reeves
利用人工合成的寡核苷酸杂交探针，从小鼠Friend细胞文库中克隆了非组蛋白染色体蛋白HMG-I或其亚型HMG-Y的编码基因。序列分析表明，1670碱基对的全长插入片段由201碱基对、富含G/C(74%)的5‘非翻译区、288碱基对的氨基酸编码序列和1182碱基对的3’非翻译区组成。小鼠HMG-I(Y)基因的96个氨基酸编码序列与已报道的人HMG-I的氨基酸序列非常相似，不同之处在于它缺少11个已报道的人类蛋白质内部氨基酸。根据基因组DNA的Southern杂交分析，单倍体小鼠基因组中的HMG-I(Y)基因似乎少于5个拷贝。这些小鼠HMG-I(Y)基因含有一个大的外显子(至少890个碱基对)，它包括大部分或全部的3‘-非翻译区；而短得多的5’-非翻译区和氨基酸编码序列被至少一个内含子打断。在快速分裂的转化细胞的总RNA提取液中检测到高水平的单一大小的HMG-I(Y)mRNAs(在小鼠细胞中约为1700个核苷酸，在人类细胞中约为2000个核苷酸)，但在缓慢分裂或不分裂的细胞中检测到的程度较小或根本没有。
A cDNA coding for the non-histone chromosomal protein HMG-I, or its isoform HMG-Y, was isolated from a murine Friend cell library using synthetic oligonucleotide hybridization probes. Sequence analysis showed that the 1670-base pair full length cDNA insert consists of a 201-base pair, G/C-rich (74%), 5'-untranslated region, a 288-base pair amino acid coding sequence, and an unusually long 1182-base pair 3'-untranslated region. The deduced 96-residue amino acid coding sequence of the murine HMG-I(Y) cDNA is very similar to the reported amino acid sequence of human HMG-I, except that it lacks 11 internal amino acids reported in the human protein. Based on Southern blot hybridization analysis of genomic DNA, there appear to be fewer than five copies of HMG-I(Y) genes in the haploid murine genome. These murine HMG-I(Y) genes contain a large (at least 890 base pairs) exon that includes most, or all, of the 3'-untranslated region; whereas the much shorter 5'-untranslated region and amino acid coding sequences are interrupted by at least one intron. A single size class (approximately 1700 nucleotides in murine cells and 2000 nucleotides in human cells) of HMG-I(Y) mRNAs was detected at high levels in total RNA extracts from rapidly dividing, transformed cells, but to a lesser extent, or not at all, in extracts from slowly or non-dividing cells.