Emulsion electrospun vascular endothelial growth factor encapsulated poly(l-lactic acid-co-ε-caprolactone) nanofibers for sustained release in cardiac tissue engineering
Emulsion electrospun vascular endothelial growth factor encapsulated poly(l-lactic acid-co-ε-caprolactone) nanofibers for sustained release in cardiac tissue engineering
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DOI：
10.1007/s10853-011-6166-4
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发表时间：
2012-04
影响因子：
4.5
通讯作者：
Lingling Tian;M. Prabhakaran;X. Ding;D. Kai;S. Ramakrishna
中科院分区：
文献类型：
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作者：
Lingling Tian;M. Prabhakaran;X. Ding;D. Kai;S. Ramakrishna
乳液静电纺丝是一种制造核壳纳米纤维的新方法，它具有多种优点，例如减轻药物的初始爆发释放，并保护掺入的药物或蛋白质的生物活性。为了开发一种有前景的心血管组织再生基质的缓释支架，我们通过乳液静电纺丝将血管内皮生长因子（VEGF）与保护剂葡聚糖或牛血清白蛋白（BSA）一起封装到聚（L-乳酸-共-ε-己内酯）（PLCL）纳米纤维的核心中。通过扫描电子显微镜测定乳液电纺支架的形貌和纤维直径，并通过激光扫描共聚焦显微镜评估核壳结构。通过乳液纺丝获得了均匀的 PLCL、PLCL-VEGF-BSA 和 PLCL-VEGF-DEX 纳米纤维，纤维直径分别在 572 ± 92、460 ± 63 和 412 ± 61 nm 范围内。评估了磷酸盐缓冲盐水中长达 672 小时（28 天）的 VEGF 释放曲线，并通过使用人骨髓来源的间充质干细胞进行细胞增殖来建立支架功能。我们的研究结果表明，含有核壳结构 PLCL 纳米纤维的乳液电纺 VEGF 通过乳液电纺核壳结构纳米纤维可控制 VEGF 的释放，并且可以成为心脏组织再生的潜在基质。
Emulsion electrospinning is a novel approach to fabricate core–shell nanofibers, and it is associated with several advantages such as the alleviation of initial burst release of drugs and it protects the bioactivity of incorporated drugs or proteins. Aiming to develop a sustained release scaffold which could be a promising substrate for cardiovascular tissue regeneration, we encapsulated vascular endothelial growth factor (VEGF) with either of the protective agents, dextran or bovine serum albumin (BSA) into the core of poly(l-lactic acid-co-ε-caprolactone) (PLCL) nanofibers by emulsion electrospinning. The morphologies and fiber diameters of the emulsion electrospun scaffolds were determined by scanning electron microscope, and the core–shell structure was evaluated by laser scanning confocal microscope. Uniform nanofibers of PLCL, PLCL–VEGF–BSA, and PLCL–VEGF–DEX with fiber diameters in the range of 572 ± 92, 460 ± 63, and 412 ± 61 nm, respectively were obtained by emulsion spinning. The release profile of VEGF in phosphate-buffered saline for up to 672 h (28 days) was evaluated, and the scaffold functionality was established by performing cell proliferations using human bone marrow derived mesenchymal stem cells. Results of our study demonstrated that the emulsion electrospun VEGF containing core–shell structured PLCL nanofibers offered controlled release of VEGF through the emulsion electrospun core–shell structured nanofibers and could be potential substrates for cardiac tissue regeneration.