S-Glycoprotein-Like Protein Regulates Defense Responses in Nicotiana Plants against Ralstonia solanacearum
S-Glycoprotein-Like Protein Regulates Defense Responses in Nicotiana Plants against Ralstonia solanacearum
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DOI：
10.1104/pp.109.148189
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发表时间：
2010-04-01
期刊：
影响因子：
7.4
通讯作者：
Kiba, Akinori
中科院分区：
文献类型：
--
作者：
Maimbo, Milimo;Ohnishi, Kouhei;Kiba, Akinori
RsRGA 4（Ralstonia solanacearum responsive gene A4）编码一种与芜菁S-位点糖蛋白（SGP）相似的多肽，以及与三裂叶牵牛（Ipomoea trifida）和蒺藜苜蓿（Medicago truncatula）的SGP样蛋白相似的多肽。因此，我们将RsRGA 4命名为NtSGLP（烟草SGP-like蛋白）和NbSGLP（其烟草本塞姆氏直向同源物）。NbSGLP在根、叶、花瓣、雌蕊群和雄蕊中表达。接种R.接种青枯菌（Rs 8107）后，对青枯菌的抗性略有增强。青枯菌（RsOE 1 -1）。通过接种RsOE 1 -1和Rs 8107的hrpY缺陷突变体诱导NbSGLP的表达。氨基环丙烷羧酸和水杨酸也诱导表达。病毒诱导的NbSGLP基因沉默增强了Rs 8107的生长。RsOE 1 -1的生长和枯萎症状的出现也加快了沉默植物。PR-1a和EREBP的表达减少，基础防御的标志物，如胼胝质沉积和减少血管流量，在NbSGLP沉默的植物中受到损害。此外，在沉默的植物中，菊苣假单胞菌、烟粉虱假单胞菌和甜瓜假单胞菌的生长也得到增强。另一方面，NbSGLP的沉默不干扰过敏反应的出现。NbSGLP以信号肽依赖的方式分泌。根癌农杆菌介导的NbSGLP的表达诱导PR-1a和EREBP的表达，胼胝质沉积，并减少血管流量。在水杨酸缺乏的N. benthamiana NahG植物。综上所述，SGLP可能在烟草属植物的基部防御诱导中起作用。
RsRGA4 (for Ralstonia solanacearum-responsive gene A4) encodes a polypeptide similar to S-locus glycoprotein (SGP) from Brassica rapa and SGP-like proteins from Ipomoea trifida and Medicago truncatula. Therefore, we designated RsRGA4 as NtSGLP (for Nicotiana tabacum SGP-like protein) and NbSGLP (its Nicotiana benthamiana ortholog). NbSGLP is expressed in root, leaf, petal, gynoecium, and stamen. Expression of NbSGLP was strongly induced by inoculation with an avirulent strain of R. solanacearum (Rs8107) and slightly enhanced by inoculation with virulent R. solanacearum (RsOE1-1). Expression of NbSGLP was induced by inoculation with an hrpY-deficient mutant of RsOE1-1 and Rs8107. Expression was also induced by aminocyclopropane carboxylic acid and salicylic acid. Virus-induced gene silencing of NbSGLP enhanced the growth of Rs8107. Growth of RsOE1-1 and appearance of wilt symptoms were also accelerated in silenced plants. Expression of PR-1a and EREBP was reduced, and markers for basal defense, such as callose deposition and reduced vascular flow, were compromised in NbSGLP-silenced plants. Moreover, growth of Pseudomonas cichorii, Pseudomonas syringae pv tabaci, and P. syringae pv mellea was also enhanced in the silenced plants. On the other hand, silencing of NbSGLP did not interfere with the appearance of the hypersensitive response. NbSGLP was secreted in a signal peptide-dependent manner. Agrobacterium tumefaciens-mediated expression of NbSGLP induced PR-1a and EREBP expression, callose deposition, and reduced vascular flow. NbSGLP-induced callose deposition and reduced vascular flow were not observed in salicylic acid-deficient N. benthamiana NahG plants. Taken together, SGLP might have a role in the induction of basal defense in Nicotiana plants.