Gut Microbiota, Glucose, Lipid, and Water-Electrolyte Metabolism in Children With Nonalcoholic Fatty Liver Disease.
Gut Microbiota, Glucose, Lipid, and Water-Electrolyte Metabolism in Children With Nonalcoholic Fatty Liver Disease.
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非酒精性脂肪肝儿童的肠道微生物群、葡萄糖、脂质和水电解质代谢
DOI：
10.3389/fcimb.2021.683743
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发表时间：
2021
影响因子：
5.7
通讯作者：
Luo J
中科院分区：
文献类型：
--
作者：
Pan X;Kaminga AC;Liu A;Wen SW;Luo M;Luo J
有证据表明，非酒精性脂肪肝（NAFLD）受肠道微生物群，葡萄糖和脂质的影响。然而，NAFLD儿童的水电解质代谢功能仍不明确。因此，本病例对照研究的目的是更好地了解这些相互作用。样本包括75名儿童，年龄在7至16岁之间，其中25名患有非酒精性脂肪肝（NAFL），25名患有非酒精性脂肪性肝炎（NASH），25名肥胖且无NAFLD。这些组按年龄、性别和体重指数进行匹配。数据于2019年6月至2019年12月在中国湖南省儿童医院收集。使用16 S核糖体RNA扩增子测序评估粪便样品中的微生物组组成。临床指标中包括12项糖脂代谢指标和6项水电解质代谢指标。结果表明，NAFLD儿童的微生物群α多样性低于其他两组，但β多样性指数高于其他两组。具体而言，抗炎和益生菌丰度（例如，粪杆菌、阿克曼氏菌和双歧杆菌）在NAFLD中显著减少，而有害细菌（例如，葡萄球菌科）增加。此外，丁酸盐产生细菌的丰度（例如，粪杆菌属、Roseburia_inulinivorans、Roseburia_inulinivorans和粪球菌属）在NASH中显著减少。这些细菌的丰度与葡萄糖、脂质和水-电解质代谢有关（例如，葡萄糖、甘油三酯、胆固醇、无机盐、全身水分等），提示NAFLD及其严重程度与糖、脂、水电解质代谢失调有关。因此，这些研究结果表明，肠道微生物组，特别是产丁酸细菌，在儿童NAFLD的发展中起着重要作用。
There is evidence that nonalcoholic fatty liver disease (NAFLD) is affected by gut microbiota, glucose, and lipid. However, the function of water-electrolyte metabolism remains undefined in children with NAFLD. Therefore, the aim of this case-control study was to better understand these interactions. The sample consisted of 75 children, aged between 7 and 16, of whom 25 had nonalcoholic fatty liver (NAFL), 25 had nonalcoholic steatohepatitis (NASH), and 25 were obese and without NAFLD. These groups were matched by age, sex, and body mass index. Data were collected between June, 2019 and December, 2019 at the Hunan Children’s Hospital, in China. Microbiome composition in fecal samples was assessed using 16S ribosomal RNA amplicon sequencing. In the clinical indices, 12 glucose and lipid metabolism indices were included, and six water-electrolyte metabolism indices were included. The results indicated that microbiomes of NAFLD children had lower alpha diversity but higher beta diversity index than the other two groups. Specifically, anti-inflammatory and probiotics abundance (e.g., Faecalibacterium, Akkermansia, and Bifidobacterium_adolescentis) was significantly decreased in NAFLD, whereas the abundance of harmful bacteria (e.g., Staphylococcaceae) was increased. Moreover, the abundance of butyrate-producing bacteria (e.g., Faecalibacterium, Roseburia_inulinivorans, Roseburia_intestinalis, and Coprococcus_comes) was significantly decreased in NASH. The abundance of these bacteria were associated with glucose, lipid, and water-electrolyte metabolism (e.g., glucose, triglyceride, cholesterol, inorganic salt, total body water, etc.), implying that the NAFLD and its severity were associated with glucose, lipid, and water-electrolyte metabolism dysbiosis. Therefore, these findings suggest that the gut microbiome, especially butyrate-producing bacteria, play an important role in the development of NAFLD in children.