A reduction of sucrose phosphate synthase (SPS) activity affects sucrose/starch ratio in leaves but does not inhibit normal plant growth in rice
A reduction of sucrose phosphate synthase (SPS) activity affects sucrose/starch ratio in leaves but does not inhibit normal plant growth in rice
复制标题
水稻蔗糖磷酸合成酶（SPS）活性的降低影响叶片中蔗糖/淀粉的比值，但不影响植株的正常生长
DOI：
10.1016/j.plantsci.2016.08.017
复制
发表时间：
2016-12-01
期刊：
影响因子：
5.2
通讯作者：
Aoki, Naohiro
中科院分区：
文献类型：
--
作者：
Hashida, Yoichi;Hirose, Tatsuro;Aoki, Naohiro
蔗糖磷酸合成酶（SPS）是植物叶片中蔗糖/淀粉比例的调节基因，对主要在叶片中积累淀粉的淀粉叶植物进行了研究。然而，SPS对水稻叶片中蔗糖/淀粉比的贡献，主要是积累蔗糖（即，糖叶），由于先前研究结果的不一致，尚未得到证实。在这项研究中，我们分析了SPS活性降低的突变株系，这些突变株系是使用Tos 17插入、RNAi和CRISPR/Cas9系统产生的。OsSPS 1基因敲除和基因敲除突变体的叶片SPS活性降低了29-46%，但叶片中的碳水化合物含量和植物生长与野生型植物没有显着差异。在OsSPS 1和OsSPS 11的双敲除突变体（sps 1/sps 11）中，叶片SPS活性降低84%导致叶片中的淀粉积累高于野生型叶片。然而，sps 1/sps 11植株生长正常，这与典型的淀粉叶植物拟南芥的SPS突变体的生长受到抑制相反。这些结果表明，SPS对水稻叶片蔗糖/淀粉比和生长的影响较小，而对淀粉叶种类的影响较小。(C)2016爱思唯尔爱尔兰有限公司版权所有。
Sucrose phosphate synthase (SPS) has been shown to mediate sucrose/starch ratio in plant leaves through studies of 'starch leaf species that mainly accumulate starch in their leaves. However, the contribution of SPS to sucrose/starch ratio in rice leaves, which mainly accumulate sucrose (i.e., 'sugar leaf), has not been confirmed due to inconsistencies in the results of previous studies. In this study, we analyzed mutant lines with reduced SPS activity, which were generated using Tos17 insertion, RNAi, and the CRISPR/Cas9 system. The knockdown and knockout mutants of OsSPS1 showed a 29-46% reduction in SPS activity in the leaves, but the carbohydrate content in the leaves and plant growth were not significantly different from those of wild-type plants. In a double knockout mutant of OsSPS1 and OsSPS1 1 (sps1/sps11), an 84% reduction in leaf SPS activity resulted in higher starch accumulation in the leaves than in the wild-type leaves. However, the sps1/sps11 plants grew normally, which is in contrast to the inhibited growth of SPS mutants of Arabidopsis thaliana, a typical starch leaf plant. These results suggest that SPS has a smaller effect on the sucrose/starch ratio in leaves and growth of rice than on starch leaf species. (C) 2016 Elsevier Ireland Ltd. All rights reserved.