Induced overexpression of mitochondrial Mn-superoxide dismutase extends the life span of adult Drosophila melanogaster.
Induced overexpression of mitochondrial Mn-superoxide dismutase extends the life span of adult Drosophila melanogaster.
复制标题
诱导线粒体锰超氧化物歧化酶的过度表达可延长成年果蝇的寿命。
DOI：
10.1093/genetics/161.2.661
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发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Tower,John
中科院分区：
文献类型：
--
作者：
Sun,Jingtao;Folk,Donna;Bradley,TimothyJ;Tower,John
基于酵母FLP重组酶的转基因系统（“FLP-out”）可以诱导成年果蝇中MnSOD酶的过表达，通过对幼果蝇的短暂热脉冲（HP），FLP-out触发了MnSOD转基因的重排和随后在整个成年寿命中的表达。对照（无HP）和过表达（HP）果蝇具有相同的遗传背景。MnSOD酶过表达的量在6个独立的转基因株系中各不相同，增加了75%。寿命的增加与酶的增加成比例。平均寿命增加了16%，有些品系增加了30-33%。最大寿命平均增加了15%，有一条线显示增加了37%。过氧化氢酶与MnSOD同时过表达没有额外的好处，与以前的观察，过氧化氢酶是目前在成年苍蝇的寿命方面过量。Cu/ZnSOD过表达也增加平均寿命和最大寿命。对于MnSOD和Cu/ZnSOD系，寿命的增加与代谢活性的降低无关，以O2消耗量为衡量标准。
A transgenic system (“FLP-out”) based on yeast FLP recombinase allowed induced overexpression of MnSOD enzyme in adultDrosophila melanogaster.With FLP-outa brief heat pulse (HP) of young, adult flies triggered the rearrangement and subsequent expression of aMnSODtransgene throughout the adult life span. Control (no HP) and overexpressing (HP) flies had identical genetic backgrounds. The amount of MnSOD enzyme overexpression achieved varied among six independent transgenic lines, with increases up to 75%. Life span was increased in proportion to the increase in enzyme. Mean life span was increased by an average of 16%, with some lines showing 30-33% increases. Maximum life span was increased by an average of 15%, with one line showing as much as 37% increase. Simultaneous overexpression of catalase with MnSOD had no added benefit, consistent with previous observations that catalase is present in excess in the adult fly with regard to life span. Cu/ZnSOD overexpression also increases mean and maximum life span. For bothMnSODandCu/ZnSODlines, increased life span was not associated with decreased metabolic activity, as measured by O2consumption.