Genetic diversity of swine influenza viruses isolated from pigs during 2000 to 2005 in Thailand.
Genetic diversity of swine influenza viruses isolated from pigs during 2000 to 2005 in Thailand.
复制标题
2000年至2005年在泰国，猪流感病毒的遗传多样性。
DOI：
10.1111/j.1750-2659.2008.00062.x
复制
发表时间：
2008-09
影响因子：
4.4
通讯作者：
Saito T
中科院分区：
文献类型：
--
作者：
Takemae N;Parchariyanon S;Damrongwatanapokin S;Uchida Y;Ruttanapumma R;Watanabe C;Yamaguchi S;Saito T
研究背景近年来的研究表明，世界范围内的猪流感病毒（SIV）存在着遗传多样性。 在泰国，自20世纪70年代末首次分离出H1N1和H3 N2亚型病毒以来，有关SIV分子特征的信息很少。我们先前的研究表明，泰国H1N1 SIV具有经典猪H1和禽样猪N1基因（Takemae et al.，Proceedings of the Options for the Control of Influenza VI. 2007;350-353）。 目的本研究对2000年至2005年分离的12株SIV进行了基因特征分析，包括H1N1、H1 N2和H3 N2亚型。 方法对分离株的8个基因片段进行全序列测定。 结果系统发育分析显示泰国SIV存在9种不同的基因型。 这些基因型来自于经典猪、禽样猪和人病毒的多次引入。泰国SIV的经典猪H1和NS、禽样猪PA和M以及人H3和N2基因中存在两个不同的亚系，表明经典猪、禽样猪和人源病毒分别两次引入泰国猪群。在泰国SIV中，禽类样猪基因的优势是显而易见的。特别是，在所有检查的泰国SIV中保留了三种禽样猪源聚合酶（PB 1、PB 2和PA）和基质基因。 结论这些观察结果可能表明，类似禽类的猪谱系的基因在猪中保持有一些优势，正如在其他地区通过多次引入建立的SIV中所看到的那样。
Background Recent studies have revealed the existence of genetic diversity in swine influenza viruses (SIVs) in the world. In Thailand, there has been a little information on the molecular characteristics of the SIVs since the first isolation of viruses of H1N1 and H3N2 subtypes in the late 1970s. Our previous study demonstrated that Thai H1N1 SIVs possessed the classical swine H1 and avian‐like swine N1 genes (Takemae et al., Proceedings of the Options for the Control of Influenza VI.2007;350–353). Objectives In the present study, we genetically characterized 12 SIVs including those of H1N1, H1N2 and H3N2 subtypes isolated between 2000 and 2005. Methods We determined the entire nucleotide sequences of the eight gene segments of those isolates. Results Phylogenetic analysis revealed the existence of nine distinct genotypes amongst the Thai SIVs. These genotypes arose from multiple introductions of classical swine, avian‐like swine and human viruses. The existence of two distinct sublineages within classical swine H1 and NS, avian‐like swine PA and M and human H3 and N2 genes of the Thai SIVs suggested that introduction of viruses of classical swine, avian‐like swine and human origins occurred twice respectively into the Thai pig population. The predominance of avian‐like swine genes amongst the Thai SIVs was evident. In particular, three polymerase (PB1, PB2 and PA) and matrix genes of avian‐like swine origin were retained in all the Thai SIVs examined. Conclusions These observations may suggest that genes of avian‐like swine lineages have some advantages to be maintained in pigs as seen in the SIVs established through multiple introductions in other regions.