Identification of TaWD40D, a wheat WD40 repeat-containing protein that is associated with plant tolerance to abiotic stresses
Identification of TaWD40D, a wheat WD40 repeat-containing protein that is associated with plant tolerance to abiotic stresses
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DOI：
10.1007/s00299-014-1717-1
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发表时间：
2015-03-01
影响因子：
6.2
通讯作者：
Li, Xia
中科院分区：
文献类型：
--
作者：
Kong, Dejing;Li, Mengjun;Li, Xia
TaWD 40 D基因是一个新发现的与小麦非生物胁迫应答有关的基因，编码WD 40家族蛋白。TaWD 40 D是植物对盐胁迫和渗透胁迫反应的正向调节因子，非生物胁迫严重影响植物生长和作物产量。含WD 40重复序列的蛋白质在蛋白质-蛋白质或蛋白质-DNA相互作用中通过充当支架分子和促进蛋白质活性而发挥关键作用。本研究从中国春小麦（Triticum aestivum L.）TaWD 40 D编码含有7个WD 40结构域的蛋白。在烟草叶肉细胞和拟南芥根细胞中的亚细胞定位表明TaWD 40 D存在于细胞质和细胞核中。拟南芥中TaWD 40 D的杂合过量表达极大地提高了植物在种子萌发和幼苗发育过程中对脱落酸（阿坝）、盐胁迫和渗透胁迫的耐受性。在过量表达TaWD 40 D的转基因株系中，SOS途径的两个基因（SOS 2和SOS 3）和ABA依赖途径和ABA非依赖途径的三阿坝基因（ABI 2、RAB 18和DREB 2A）的表达模式在处理下发生了改变。值得注意的是，ABI 2表达的基础水平在TaWD 40 D过表达系中显著增加。TaWD 40 D基因沉默导致小麦相对含水量降低，生长势减弱。我们的研究结果表明，TaWD 40 D功能作为一个积极的调节植物对盐胁迫和渗透胁迫的反应，可用于作物耐逆性的遗传改良。
TaWD40D that encodes a member of WD40 family proteins is a novel gene involved in the wheat response to abiotic stress. TaWD40D functions as a positive regulator of plant responses to salt stress and osmotic stress in plant.Abiotic stresses can severely affect plant growth and crop productivity. WD40 repeat-containing proteins play a key role in protein-protein or protein-DNA interactions by acting as scaffolding molecules and promoting protein activity. In this study, a stress-inducible gene, TaWD40D, was identified from Chinese spring wheat (Triticum aestivum L.). TaWD40D encodes a protein containing seven WD40 domains. Subcellular localization in Nicotiana benthamiana mesophyll cells and Arabidopsis root cells showed the presence of TaWD40D in the cytoplasm and nucleus. Heterologous overexpression of TaWD40D in Arabidopsis greatly increased plant tolerance to abscisic acid (ABA), salt stress, and osmotic stress during seed germination and seedling development. The expression patterns of two genes from the SOS pathway (SOS2 and SOS3) and three ABA genes (ABI2, RAB18 and DREB2A) functioning in ABA-dependent and ABA-independent pathways were altered in the transgenic lines overexpressing TaWD40D under the treatments. Notably, the basal level of the ABI2 expression was substantially increased in the TaWD40D overexpression lines. The down-regulation of TaWD40D in wheat by virus-induced gene silencing resulted in a decreased relative water content and less vigorous growth compared to non-silenced lines. Our results suggest that TaWD40D functions as a positive regulator of plant responses to salt stress and osmotic stress that could be utilized for the genetic improvement of stress tolerance in crop plants.