Gas-Foaming Calcium Phosphate Cement Scaffold Encapsulating Human Umbilical Cord Stem Cells
Gas-Foaming Calcium Phosphate Cement Scaffold Encapsulating Human Umbilical Cord Stem Cells
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DOI：
10.1089/ten.tea.2011.0267
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发表时间：
2012-04-01
影响因子：
4.1
通讯作者：
Xu, Hockin H. K.
中科院分区：
文献类型：
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作者：
Chen, Wenchuan;Zhou, Hongzhi;Xu, Hockin H. K.
组织工程方法有望满足日益增长的骨再生需求。磷酸钙水泥（CPC）可以通过注射和自固化形成具有良好骨导电性的支架。本研究的目的是开发一种含有海藻酸纤维蛋白微珠的大孔CPC-壳聚糖-纤维结构，包封人脐带间充质干细胞（hUCMSCs），并研究hUCMSC从降解微珠中的释放和在多孔CPC结构中的增殖。hucmsc包封的微珠完全包裹在CPC膏体中，气体发泡多孔剂在CPC中形成大孔，为培养基提供通道。增加CPC中孔隙素含量可显著提高细胞活力，从0%孔隙素CPC中活细胞的49%提高到15%孔隙素CPC中活细胞的86%。海藻酸纤维蛋白微球在第7天开始降解并释放细胞。释放的细胞开始在大孔CPC结构内增殖。CPC内的活细胞数从第1天的270个/mm(2)增加到第21天的350个/mm(2)。采用气发泡法，CPC的孔隙体积分数从46.8%提高到78.4%，大孔尺寸约为100 ~ 400mm。cpc -壳聚糖纤维支架在15%孔隙率下的强度为3.8 MPa，接近报道的松质骨的3.5 MPa。综上所述，我们开发了一种含有可降解海藻酸纤维蛋白微珠的新型气体发泡大孔CPC结构，用于包裹hUCMSCs。包裹在多孔CPC结构内的细胞具有良好的活力。CPC中可降解的微珠迅速释放细胞，随着时间的推移，细胞在多孔CPC中增殖。具有海藻酸纤维蛋白微珠的自固化强CPC干细胞递送在骨组织工程应用中很有前景。
Tissue engineering approaches are promising to meet the increasing need for bone regeneration. Calcium phosphate cement (CPC) can be injected and self-set to form a scaffold with excellent osteoconductivity. The objectives of this study were to develop a macroporous CPC-chitosan-fiber construct containing alginate-fibrin microbeads encapsulating human umbilical cord mesenchymal stem cells (hUCMSCs) and to investigate hUCMSC release from the degrading microbeads and proliferation inside the porous CPC construct. The hUCMSC-encapsulated microbeads were completely wrapped inside the CPC paste, with the gas-foaming porogen creating macropores in CPC to provide for access to culture media. Increasing the porogen content in CPC significantly increased the cell viability, from 49% of live cells in CPC with 0% porogen to 86% of live cells in CPC with 15% porogen. The alginate-fibrin microbeads started to degrade and release the cells inside CPC at 7 days. The released cells started to proliferate inside the macroporous CPC construct. The live cell number inside CPC increased from 270 cells/mm(2) at 1 day to 350 cells/mm(2) at 21 days. The pore volume fraction of CPC increased from 46.8% to 78.4% using the gas-foaming method, with macropore sizes of approximately 100 to 400 mm. The strength of the CPC-chitosan-fiber scaffold at 15% porogen was 3.8 MPa, which approximated the reported 3.5 MPa for cancellous bone. In conclusion, a novel gas-foaming macroporous CPC construct containing degradable alginate-fibrin microbeads was developed that encapsulated hUCMSCs. The cells had good viability while wrapped inside the porous CPC construct. The degradable microbeads in CPC quickly released the cells, which proliferated over time inside the porous CPC. Self-setting, strong CPC with alginate-fibrin microbeads for stem cell delivery is promising for bone tissue engineering applications.