Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells
Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells
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DOI：
10.1016/j.bbrc.2005.05.030
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发表时间：
2005-07-08
影响因子：
3.1
通讯作者：
Kawano, T
中科院分区：
文献类型：
--
作者：
Lin, C;Yu, YW;Kawano, T
此前，在表达 Ca2+ 监控发光蛋白、水母发光蛋白和拟南芥中新分离的假定植物 Ca2+ 通道蛋白的烟草细胞中评估了 A1 离子对钙信号传导的影响。 AtTPC1（双孔通道 1）。 TPC1 通道被证明是唯一已知对 At 敏感的通道，并且它们对活性氧和隐菌素（一种真菌激发子蛋白）有反应。因此，有人认为 TPC1 通道参与钙信号传导，从而导致植物防御机制的发展。然后，有人提出使用At作为TPC1型植物钙通道的特异性抑制剂。在这里，我们使用表达水母发光蛋白的转基因烟草BY-2细胞，报告了支持A1敏感信号通路参与的证据，该信号通路需要TPC1型通道依赖性Ca2+流入来响应水杨酸（一种关键的植物防御诱导剂），但不响应从稻瘟病真菌稻瘟病菌细胞壁制备的激发子。此外，还测试了 A1 敏感 Ca2+ 通道对冷休克反应的参与情况。数据表明，这里使用的引发剂通过A1不敏感途径诱导Ca2+流入，而水杨酸和冷休克通过At敏感机制刺激Ca2+流入。 (c) 2005 Elsevier Inc. 保留所有权利。
Previously, effect of A1 ions on calcium signaling was assessed in tobacco cells expressing a Ca2+ -monitoring luminescent protein, aequorin and a newly isolated putative plant Ca2+ channel protein from Arabidopsis thaliana. AtTPC1 (two-pore channel 1). TPC1 channels were shown to be the only channel known to be sensitive to At and they are responsive to reactive oxygen species and cryptogein, a fungal elicitor protein. Thus, involvement of TPC1 channels in calcium signaling leading to development of plant defense mechanism has been suggested. Then, the use of At as a specific inhibitor of TPC1-type plant calcium channels has been proposed. Here, using transgenic tobacco BY-2 cells expressing aequorin, we report on the evidence in support of the involvement of A1-sensitive signaling pathway requiring TPC1-type channel-dependent Ca2+ influx in response to salicylic acid, a key plant defense-inducing agent, but not to an elicitor prepared from the cell wall of rice blast disease fungus Magnaporthe grisea. In addition, involvement of A1-sensitive Ca2+ channels in response to cold shock was also tested. The data suggested that the elicitor used here induces the Ca2+ influx via A1-insensitive path, while salicylic acid and cold-shock-stimulate the influx of Ca2+ via At-sensitive mechanism. (c) 2005 Elsevier Inc. All rights reserved.