SPECIFICITY OF PYOVERDINE-MEDIATED IRON UPTAKE AMONG FLUORESCENT PSEUDOMONAS STRAINS
SPECIFICITY OF PYOVERDINE-MEDIATED IRON UPTAKE AMONG FLUORESCENT PSEUDOMONAS STRAINS
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DOI：
10.1128/jb.170.10.4865-4873.1988
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发表时间：
1988-10-01
影响因子：
3.2
通讯作者：
MEYER, JM
中科院分区：
文献类型：
--
作者：
HOHNADEL, D;MEYER, JM
绿脓菌荧光素介导的铁转运的7个荧光假单胞菌菌株属于不同的物种。对于所有菌株，将细胞或细胞外膜和铁（III）-绿脓菌荧光素组合与其在摄取、结合和交叉喂养实验中的同源对应物进行比较。对于四种菌株（恶臭假单胞菌ATCC 12633、荧光假单胞菌W、荧光假单胞菌ATCC 17400和托氏假单胞菌NCPPB 2192），绿脓菌荧光素介导的铁转运似乎具有严格的菌株特异性;绿脓菌荧光素促进铁饥饿细胞的铁摄取，以及铁吡咯烷与此类细胞的纯化外膜的结合仅在同源系统的情况下有效。然而，特别是对于荧光假单胞菌ATCC 17400，在液体或固体培养物中的交叉喂养测定与摄取和结合测定相比产生一些差异，表明生长实验最不可能产生关于绿脓菌荧光素介导的铁转运的特异性的正确信息。对于其他三种菌株（荧光假单胞菌ATCC 13525、绿针假单胞菌ATCC 9446和铜绿假单胞菌ATCC 15692），通过摄取、结合和交叉饲喂实验证明了交叉反应性。在试图确定哪些部分的铁转运系统负责的特异性，在绿脓菌荧光素的氨基酸组成的差异，以及在铁敏感的外膜蛋白模式的7个菌株的水平上观察到的差异，进行了讨论。
Pyoverdine-mediated iron transport was determined for seven fluorescent Pseudomonas strains belonging to different species. For all strains, cell or cell outer membrane and iron(III)-pyoverdine combinations were compared with their homologous counterparts in uptake, binding, and cross-feeding experiments. For four strains (Pseudomonas putida ATCC 12633, Pseudomonas fluorescens W, P. fluorescens ATCC 17400, and Pseudomonas tolaasii NCPPB 2192), the pyoverdine-mediated iron transport appeared to be strictly strain specific; pyoverdine-facilitated iron uptake by iron-starved cells and binding of ferripyroverdine to the purified outer membranes of such cells were efficient only in the case of the homologous systems. Cross-feeding assays, in liquid or solid cultures, resulted, however, especially for P. fluorescens ATCC 17400, in some discrepancies compared with uptake and binding assays, suggesting that growth experiments are the least likely to yield correct information on specificity of the pyoverdine-mediated iron transport. For the three other strains (P. fluorescens ATCC 13525, P. chlororaphis ATCC 9446, and P. aeruginosa ATCC 15692), cross-reactivity was demonstrated by the uptake, binding, and cross-feeding experiments. In an attempt to determine which parts of the iron transport system were responsible for the specificity, the differences in amino acid composition of the pyoverdines, together with the differences observed at the level of the iron-sensitive outer membrane protein pattern of the seven strains, are discussed.