Down-regulation of genes coding for core RNAi components and disease resistance proteins via corresponding microRNAs might be correlated with successful Soybean mosaic virus infection in soybean
Down-regulation of genes coding for core RNAi components and disease resistance proteins via corresponding microRNAs might be correlated with successful Soybean mosaic virus infection in soybean
复制标题
通过相应的 microRNA 下调编码核心 RNAi 成分和抗病蛋白的基因可能与大豆花叶病毒感染大豆的成功相关。
DOI：
10.1111/mpp.12581
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发表时间：
2018-04-01
影响因子：
4.9
通讯作者：
Wuriyanghan, Hada
中科院分区：
文献类型：
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作者：
Bao, Duran;Ganbaatar, Oyunchuluun;Wuriyanghan, Hada
植物通过几种不同的防御机制来保护自己免受病毒感染。RNA干扰（RNA interference，RNAi）是一种重要的抗病毒机制，它需要AGO（Argonaute）和Dicer/DCL（Dicer-like）蛋白参与。免疫原性触发免疫（ETI）是由抗性基因介导的一种抗病毒机制，其中大多数抗性基因编码富含亮氨酸重复序列（NBS-LRR）家族蛋白。microRNAs（miRNAs）在植物中起着重要的调控作用，包括对宿主防御的调控。大豆花叶病毒（SMV）是大豆中最常见的病毒，在这项工作中，我们通过微阵列分析在SMV敏感的大豆品系中鉴定了几十个SMV响应的miRNA。在上调的miRNAs中，miR 168 a、miR 403 a、miR 162 b和miR 1515 a分别预测性调控AGO 1、AGO 2、DCL 1和DCL 2的表达，miR 1507 a、miR 1507 c和miR 482 a预测性调控多个NBS-LRR家族抗病基因的表达。通过瞬时表达试验和RNA连接酶介导的cDNA末端快速扩增（RLM-RACE）实验验证了这7种miRNA对靶基因表达的调节作用。在SMV感染后的不同时间点，AGO 1、DCL 1、DCL 2和5个NBS-LRR家族基因的转录水平被抑制，而相应的miRNA水平在这些相同的时间点被上调。此外，通过短串联靶模拟物（STTM）技术抑制miR 1507 a、miR 1507 c、miR 482 a、miR 168 a和miR 1515 a降低了SMV在大豆中的感染效率。我们的研究结果表明，SMV可以通过下调几个RNAi途径基因和NBS-LRR家族抗性基因，通过诱导其相应的miRNA水平的积累来抵消大豆的防御反应。
Plants protect themselves from virus infections by several different defence mechanisms. RNA interference (RNAi) is one prominent antiviral mechanism, which requires the participation of AGO (Argonaute) and Dicer/DCL (Dicer-like) proteins. Effector-triggered immunity (ETI) is an antiviral mechanism mediated by resistance (R) genes, most of which encode nucleotide-binding site-leucine-rich repeat (NBS-LRR) family proteins. MicroRNAs (miRNAs) play important regulatory roles in plants, including the regulation of host defences. Soybean mosaic virus (SMV) is the most common virus in soybean and, in this work, we identified dozens of SMV-responsive miRNAs by microarray analysis in an SMV-susceptible soybean line. Amongst the up-regulated miRNAs, miR168a, miR403a, miR162b and miR1515a predictively regulate the expression of AGO1, AGO2, DCL1 and DCL2, respectively, and miR1507a, miR1507c and miR482a putatively regulate the expression of several NBS-LRR family disease resistance genes. The regulation of target gene expression by these seven miRNAs was validated by both transient expression assays and RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) experiments. Transcript levels for AGO1, DCL1, DCL2 and five NBS-LRR family genes were repressed at different time points after SMV infection, whereas the corresponding miRNA levels were up-regulated at these same time points. Furthermore, inhibition of miR1507a, miR1507c, miR482a, miR168a and miR1515a by short tandem target mimic (STTM) technology compromised SMV infection efficiency in soybean. Our results imply that SMV can counteract soybean defence responses by the down-regulation of several RNAi pathway genes and NBS-LRR family resistance genes via the induction of the accumulation of their corresponding miRNA levels.