Simultaneous determination of major phytocannabinoids, their main metabolites, and common synthetic cannabinoids in urine samples by LC-MS/MS
Simultaneous determination of major phytocannabinoids, their main metabolites, and common synthetic cannabinoids in urine samples by LC-MS/MS
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采用 LC-MS/MS 同时测定尿液样品中的主要植物大麻素、其主要代谢物和常见合成大麻素
DOI：
10.1016/j.jchromb.2016.08.002
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发表时间：
2016-10-15
影响因子：
3
通讯作者：
Jiang, Yan
中科院分区：
文献类型：
--
作者：
Dong, Xiaoru;Li, Liliang;Jiang, Yan
同时测定主要植物大麻素（THC、CBD、CBN）及其主要代谢物（11-OH-THC、THC-COOH、THC-COOH-葡萄糖醛酸）和常见合成大麻素（HU-210、JWH-018、JWH-073、JWH-250）仍然是法医毒理学中的一个问题。本研究开发了一种液相色谱-串联质谱（LC-MS/MS）方法，可同时检测人尿液样本中的上述10种分析物。色谱分离在 ACQUITY UPLC (R) BEH Phenyl 1.7 muM (2.1 x 100mm) 柱上进行，使用由 0.1% 甲酸水溶液和乙腈组成的流动相，以梯度洗脱模式，流速为 0.3 mL/min。所有分析物的检测限 (LOD) 和定量限 (LOQ) 分别为 0.01-0.5 ng/mL 和 0.05-1 ng/mL。对于植物大麻素、其主要代谢物和 HU-210，该测定在 LOQ 至 100 ng/mL 范围内呈线性；对于 JWH-250、JWH-018 和 JWH-073，该测定在 0.05 至 50 ng/mL 范围内呈线性。提取回收率超过50%，基质效应在59.4%~100.1%之间。准确度和精密度分别为
Simultaneous determination of major phytocannabinoids (THC, CBD, CBN), their main metabolites (11-OH-THC, THC-COOH, THC-COOH-glucuronide) and common synthetic cannabinoids (HU-210, JWH-018, JWH-073, JWH-250) remains an issue in forensic toxicology. The present study has developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to simultaneously detect the above 10 analytes in human urine samples. The chromatographic separation was performed on an ACQUITY UPLC (R) BEH Phenyl 1.7 mu M (2.1 x 100mm) column, using a mobile phase consisting of 0.1% formic acid in water and acetonitrile at a flow rate of 0.3 mL/min in gradient elution mode. The limit of detection (LOD) and limit of quantification (LOQ) of all analytes were 0.01-0.5 ng/mL and 0.05-1 ng/mL, respectively. The assay was linear from LOQ to 100 ng/mL for phytocannabinoids, their main metabolites and HU-210, and from 0.05 to 50 ng/mL for JWH-250, JWH-018 and JWH-073. The extraction recoveries were over 50% and the matrix effects were between 59.4% and 100.1%. The accuracy and precision were