NMR Solution Structures of δ-Conotoxin EVIA from Conus ermineus That Selectively Acts on Vertebrate Neuronal Na+ Channels*[boxs]
NMR Solution Structures of δ-Conotoxin EVIA from Conus ermineus That Selectively Acts on Vertebrate Neuronal Na+ Channels*[boxs]
复制标题
斑芋螺 δ-芋螺毒素 EVIA 的 NMR 溶液结构，选择性作用于脊椎动物神经元 Na+ 通道*[方框]
DOI：
--
复制
发表时间：
2004
影响因子：
4.8
通讯作者：
J. Lancelin
中科院分区：
文献类型：
--
作者：
L. Volpon;H. Lamthanh;J. Barbier;N. Gilles;J. Molgó;A. Ménez;J. Lancelin
δ-Conotoxin EVIA, from Conus ermineus, is a 32-residue polypeptide cross-linked by three disulfide bonds forming a four-loop framework. δ-Conotoxin EVIA is the first conotoxin known to inhibit sodium channel inactivation in neuronal membranes from amphibians and mammals (subtypes rNav1.2a, rNav1.3, and rNav1.6), without affecting rat skeletal muscle (subtype rNav1.4) and human cardiac muscle (subtype hNav1.5) sodium channel (Barbier, J., Lamthanh, H., Le Gall, F., Favreau, P., Benoit, E., Chen, H., Gilles, N., Ilan, N., Heinemann, S. F., Gordon, D., Ménez, A., and Molgó, J. (2004) J. Biol. Chem. 279, 4680-4685). Its structure was solved by NMR and is characterized by a 1:1 cis/trans isomerism of the Leu12-Pro13 peptide bond in slow exchange on the NMR time scale. The structure of both cis and trans isomers could be calculated separately. The isomerism occurs within a specific long disordered loop 2, including residues 11-19. These contribute to an important hydrophobic patch on the surface of the toxin. The rest of the structure matches the “inhibitor cystine-knot motif” of conotoxins from the “O superfamily” with a high structural order. To probe a possible functional role of the Leu12-Pro13 cis/trans isomerism, a Pro13 → Ala δ-conotoxin EVIA was synthesized and shown to exist only as a trans isomer. P13A δ-conotoxin EVIA was estimated only two times less active than the wild-type EVIA in binding competition to rat brain synaptosomes and when injected intracerebroventricularly into mice.
登录
查看更多内容
影响因子：
5.6
作者：
Eyles, SJ;Gierasch, LM
通讯作者：
Gierasch, LM
影响因子：
5.6
作者：
STEWART, DE;SARKAR, A;WAMPLER, JE
通讯作者：
WAMPLER, JE
影响因子：
2.9
作者：
OLIVERA, BM;CRUZ, LJ;RIVIER, J
通讯作者：
RIVIER, J
来自纹状芋螺毒液的新型 α 和 omega 芋螺毒素。
影响因子：
2.9
作者：
Ramilo,CA;Zafaralla,GC;Nadasdi,L;Hammerland,LG;Yoshikami,D;Gray,WR;Kristipati,R;Ramachandran,J;Miljanich,G;Olivera,BM
通讯作者：
Olivera,BM
DOI：
10.1034/j.1399-3011.2003.00048.x
发表时间：
2003-04
期刊：
The journal of peptide research : official journal of the American Peptide Society
影响因子：
--
作者：
R. D. Dela Cruz;F. Whitby;O. Buczek;G. Bulaj
通讯作者：
R. D. Dela Cruz;F. Whitby;O. Buczek;G. Bulaj