Growth reduction of Microcystis aeruginosa by clay ball elution solution
Growth reduction of Microcystis aeruginosa by clay ball elution solution
复制标题
粘土球洗脱液抑制铜绿微囊藻的生长
DOI：
10.1016/j.clay.2018.06.018
复制
发表时间：
2018
影响因子：
5.6
通讯作者：
Masanobu Mori
中科院分区：
文献类型：
--
作者：
Tsukasa Ito;Katsuyuki Okabe;Masanobu Mori
粘土是一种天然材料，其通过河流到河口的运输受到人造结构的影响。我们假设从粘土中洗脱出的化学物质会影响水华细菌。为了评估这一假设，我们创造了一个环境，在这个环境中，使用粘土球从粘土中分离细菌。当微囊藻与粘土球一起孵育时，对数生长期后期和之后的生长速率比不加粘土球的微囊藻降低了三分之二。在中性pH条件下，粘土球对氨氮和磷的吸附作用并不是导致其生长下降的主要原因。在由粘土球浸泡液、水泥球浸泡液、硅酸盐溶液和蒸馏水组成的铜绿微囊藻（MA）培养基中进行培养，结果表明，在由粘土球浸泡液组成的MA培养基中，铜绿微囊藻的生长显著降低，而在其他溶液中，铜绿微囊藻的生长没有差异。结果表明，所有溶液对微小曲壳藻的生长均无不良影响。这些结果表明，微囊藻的生长减少是由于硅酸盐和其他化合物从粘土球洗脱的综合作用。这些发现可能会提高我们对蓝藻水华的理解，并加强水管理。
Clay is a natural material, and its transportation via rivers to estuaries is influenced by man-made structures. We hypothesized that chemicals eluted from clay affect bloom bacteria. To evaluate this hypothesis, we created an environment in which bacteria were separated from clay using clay balls. WhenMicrocystisaeruginosawas incubated with clay balls, the growth rate at and after the late exponential phase decreased by two-thirds compared to that ofMicrocystisincubated without a clay ball. The clay ball adsorbed ammonia and phosphorus at neutral pH, but this was not the main reason for the growth reduction. Incubation inMicrocystis aeruginosa(MA) medium made of a clay ball-soaked solution, a cement ball-soaked solution, a silicate solution, and distilled water revealed that the growth ofMicrocystiswas significantly lower in the MA medium made of the clay ball-soaked solution, whereas there was no difference in growth among the other solutions. None of the solutions exerted a negative influence on the growth ofAchnanthidium minutissimum. These results suggest that the growth reduction ofMicrocystiswas due to a combined effect by silicate and other chemical compounds eluted from the clay ball. These findings may improve our understanding of cyanobacterial blooms and enhance water management.