Complete genome sequence of viola mottle virus, revealing its synonymous relationship to tulip virus X
Complete genome sequence of viola mottle virus, revealing its synonymous relationship to tulip virus X
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DOI：
10.1007/s00705-021-05129-4
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发表时间：
2021-06-07
影响因子：
2.7
通讯作者：
Yamaji, Yasuyuki
中科院分区：
文献类型：
--
作者：
Matsumoto, Oki;Miyazaki, Akio;Yamaji, Yasuyuki
1977年在北方意大利发现了一种名为“紫罗兰斑驳病毒”（Viola mottle virus，VMoV）的病毒。根据其形态学、血清学和生物学特性，该病毒已被暂时分类为马铃薯X病毒属的成员。然而，由于VMoV的遗传信息从未报道过，因此该病毒的分类地位尚不清楚。在这里，我们报告的第一个完整的基因组序列的VMoV，以澄清其分类地位。它的基因组RNA长6，052个核苷酸，不包括3 '-末端的poly（A）尾，并且具有五个马铃薯X病毒典型的开放阅读框（ORF）。在马铃薯X病毒中，VMoV与郁金香X病毒（TVX）的同源性最高，ORF 1的核苷酸同源性为81.1-81.2%，氨基酸同源性为90.4-90.7%，ORF 5的核苷酸同源性为82.9-83.5%，氨基酸同源性为93.2-95.2%。这些值远高于该属的种划分阈值。系统发育分析还表明，VMoV嵌套在进化枝TVX分离物。这些数据表明，VMoV和TVX是同一物种的成员。
Viola mottle virus (VMoV) was discovered in Viola odorata showing symptoms of reduced growth, leaf mottling, and whitish stripes on flowers in northern Italy in 1977. This virus has been provisionally classified as a member of the genus Potexvirus based on its morphological, serological, and biological characteristics. However, since genetic information of VMoV has never been reported, the taxonomic status of this virus is unclear. Here, we report the first complete genome sequence of VMoV to clarify its taxonomic position. Its genomic RNA is 6,052 nucleotides long, excluding the 3 '-terminal poly(A) tail, and has five open reading frames (ORFs) typical of potexviruses. Among potexviruses, VMoV showed the most similarity to tulip virus X (TVX) with 81.1-81.2% nucleotide and 90.4-90.7% amino acid sequence identity in ORF1 and 82.9-83.5% nucleotide and 93.2-95.2% amino acid sequence identity in ORF5. These values are much higher than the species demarcation threshold for the genus. Phylogenetic analysis also indicated that VMoV is nested within the clade of TVX isolates. These data demonstrate that VMoV and TVX are members of the same species.