Substance P enhances expression of lipopolysaccharide-induced inflammatory factors in dental pulp cells
Substance P enhances expression of lipopolysaccharide-induced inflammatory factors in dental pulp cells
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DOI：
10.1097/00004770-200411000-00005
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发表时间：
2004-11-01
影响因子：
4.2
通讯作者：
Torii, M
中科院分区：
文献类型：
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作者：
Tokuda, M;Miyamoto, R;Torii, M
为了研究P物质(SP)与牙髓炎症的关系，我们研究了SP对人牙髓细胞培养上炎性因子基因表达的影响。通过逆转录聚合酶链式反应，我们发现中间普氏杆菌(LPS)诱导SP和SP受体mRNAs的表达，而生长抑素抑制内毒素诱导的SP mRNAs的表达。我们还发现，SP能增强内毒素诱导的核因子-kappaB结合活性。此外，SP还可诱导环氧合酶-2和白细胞介素10受体mRNAs的表达。相反，SP可抑制干扰素-γ受体mRNA的表达。这些结果提示，SP可能在牙髓组织对病原菌的免疫应答中起调节作用。
To examine how substance P (SP) is related with dental pulp inflammation, we examined the effects of SP on expression of genes for inflammatory factors in human dental pulp cell cultures. Using reverse transcriptase-polymerase chain reaction, we found that Prevotella intermedia lipopolysaccharide (LPS) induced expression of SP and SP-receptor mRNAs, and that somatostatin inhibited the LPS-induced expression of SP mRNA. We also found that SP enhanced LPS-induced stimulation of NF-kappaB binding activity. In addition, SP induced expression of cyclooxygenase-2 and interleukin-10 receptor mRNAs. In contrast, SP inhibited expression of interferon-gamma receptor mRNA. These results suggest that SP may play a regulatory role in the immunological response of dental pulp tissue to pathogenic bacteria.