Occult HBV Infection May Be Transmitted through Close Contact and Manifest as an Overt Infection.
Occult HBV Infection May Be Transmitted through Close Contact and Manifest as an Overt Infection.
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DOI：
10.1371/journal.pone.0138552
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Fang ZL
中科院分区：
文献类型：
--
作者：
Hu LP;Liu DP;Chen QY;Harrison TJ;He X;Wang XY;Li H;Tan C;Yang QL;Li KW;Fang ZL
通过输血、器官移植和血液透析传播隐匿性HBV感染（OBI）的重要性已被广泛认识。然而，关于通过密切接触传播OBI的数据仍然有限。在这项研究中，血清样本从一个孩子和他的父母。该名儿童在出生时接受了标准的疫苗接种方案，并产生了保护性抗体。检测血清HBV血清学标志物。用巢式PCR方法检测HBV DNA，克隆扩增子，并对其序列进行系统发育分析。结果显示，父母双方都有隐性感染，而孩子有显性感染。分别对来自父亲、母亲和儿子的12个、11个和9个克隆进行了测序。父亲为adrq+、ayw1、ayw和ayr，母亲为ayw1、adw2和adwq+，儿子为adrq+。在父本中检测到基因型B、C2亚型和1个重组子，在母本中检测到基因型B、C5亚型和3个重组子。亚型C2是在儿童中鉴定的唯一基因型。系统发育树显示，所有孩子的序列和父亲的大部分序列聚在一起。然而，没有母亲的序列与孩子的序列聚类。来自孩子和他父亲的表面基因具有相同的氨基酸替换模式（T118K、T123N和G145A）。我们的结论是，父亲是儿子的HBV感染的来源，这表明隐匿性HBV感染可能通过密切接触传播，并表现为显性感染。
The importance of transmission of occult HBV infection (OBI) via transfusion, organ transplantation and hemodialysis has been widely recognized. However, data regarding the transmission of OBI through close contact remain limited. In this study, serum samples were obtained from a child and his parents. The child had received the standard vaccination regimen at birth and produced protective antibody. Sera were tested for HBV serological markers. Nested PCR assays were used to detect HBV DNA and the amplicons were cloned and their sequences subjected to phylogenetic analysis. The results showed that both parents had occult infections while the child had an overt infection. Twelve, eleven and nine clones, from the father, mother and son, respectively, were sequenced. Serotypes adrq+, ayw1, ayw and ayr were found in the father and ayw1, adw2 and adwq+ in the mother; adrq+ was the only serotype in son. Genotype B, subgenotype C2 and a recombinant were identified in the father and genotype B, subgenotype C5 and three recombinants were found in the mother. Subgenotype C2 was the only genotype identified in the child. A phylogenetic tree showed that all of the child’s sequences and most of the father’s sequences clustered together. However, none of mother’s sequences clustered with those of the child. The surface gene from the child and his father had the same amino acid substitution pattern (T118K, T123N and G145A). We concluded that the father was the source of the son’s HBV infection, suggesting that occult HBV infection may be transmitted through close contact and manifest as an overt infection.