Normal phase liquid chromatography-electrospray ionization tandem mass spectrometry analysis of phospholipid molecular species in blood mononuclear cells: application to cystic fibrosis
Normal phase liquid chromatography-electrospray ionization tandem mass spectrometry analysis of phospholipid molecular species in blood mononuclear cells: application to cystic fibrosis
复制标题
DOI：
10.1016/j.jchromb.2004.07.001
复制
发表时间：
2004-10-25
影响因子：
3
通讯作者：
Frega, NG
中科院分区：
文献类型：
--
作者：
Malavolta, M;Bocci, F;Frega, NG
使用HPLC与质谱仪在线耦合是一个非常强大的工具，以分析完整的PLs分子种类（PMS），而无需衍生化，从而降低了伪影形成的风险。建立了人血单个核细胞PMS组成的正相高效液相色谱-电喷雾串联质谱法。该方法用于描述7名CF受试者和7名年龄匹配的健康受试者的PMS特征。对磷脂酰乙醇胺（PE）、磷脂酰乙醇胺（pPE）、磷脂酰肌醇（PI）、磷脂酰丝氨酸（PS）、磷脂酰胆碱（PC）和鞘磷脂（Sph）等140多种磷脂分子进行了鉴定和比较。两组之间的差异在pPE（p16：0/22：6）、pPE（p18：0/22：6）、PE（16：0/20：4）和PC（16：0/18：2）中发现，CF受试者的pPE（p16：0/22：6）、PE（16：0/20：4）和PC（16：0/18：2）显著较低，而PC（16：0/16：1）在CF受试者中显著较高。(C)2004 Elsevier B. V.保留所有权利。
The use of HPLC coupled on-line with a mass spectrometer is a very powerful tool in order to analyze intact PLs molecular species (PMS) without the need of derivatization, thus decreasing the risk of artifacts formation. A normal-phase HPLC-ESI-MS-MS method has been developed in order to study the human blood mononuclear cell PMS composition. This method was applied to characterize PMS from seven CF subjects and from seven age-matched healthy subjects. More than 140 phospholipid molecular species from phosphatidylethanolamine (PE), plasmalogen phosphatidylethanolamine (pPE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC) and sphingomyelin (Sph) were identified and compared. Differences between the two groups were found in pPE (p16:0/22:6), pPE (p18:0/22:6), PE (16:0/20:4) and PC (16:0/18:2) which were significantly lower in CF subjects and in PC (16:0/16: 1) which was significantly higher in CF subjects. (C) 2004 Elsevier B.V. All rights reserved.