Autoantibodies against folate receptors in women with a pregnancy complicated by a neural-tube defect
Autoantibodies against folate receptors in women with a pregnancy complicated by a neural-tube defect
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DOI：
10.1056/nejmoa031145
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发表时间：
2004-01-08
影响因子：
158.5
通讯作者：
Quadros, EV
中科院分区：
文献类型：
--
作者：
Rothenberg, SP;da Costa, MP;Quadros, EV
背景：在没有临床叶酸缺乏症的情况下，围受孕期补充叶酸可降低妇女生育神经管缺陷婴儿的风险。由于抗血清叶酸受体诱导大鼠胚胎吸收和畸形，我们假设，对妇女叶酸受体的自身抗体可能与妊娠并发神经管deficiency.Methods：血清从12名妇女谁是或已怀孕的胎儿与神经管deficiency.Methods：从24名对照妇女（20与当前或以前的正常妊娠和4谁是nulligravid）进行了分析，通过培养与人胎盘叶酸受体放射性标记的[H-3]叶酸自身抗体。结果：12例受影响妊娠妇女（指标组）和20例对照组（对照组）血清中分别有9例和2例含有抗叶酸受体自身抗体（P < 0.05），其中9例血清中含有抗叶酸受体自身抗体（P < 0.05），另2例血清中含有抗叶酸受体自身抗体（P < 0.05）。
Background:In the absence of clinical folate deficiency, periconceptional supplementation with folic acid reduces a woman's risk of having an infant with a neural-tube defect. Since antiserum to folate receptors induces embryo resorption and malformations in rats, we hypothesized that autoantibodies against folate receptors in women may be associated with pregnancy complicated by a neural-tube defect.Methods:Serum from 12 women who were or had been pregnant with a fetus with a neural-tube defect and from 24 control women (20 with current or prior normal pregnancies and 4 who were nulligravid) was analyzed for autoantibodies by incubation with human placental folate receptors radiolabeled with [H-3]folic acid. The properties of these autoantibodies were characterized by incubating serum and the autoantibodies isolated from serum with placental membranes, ED27 cells, and KB cells, which express the folate receptors.Results:Serum from 9 of 12 women with a current or previous affected pregnancy (index subjects) and 2 of 20 control subjects contained autoantibodies against folate receptors (P