FREEZE-FRACTURE STUDIES OF CYTOPLASMIC INCLUSIONS OCCURRING IN EXPERIMENTAL LIPIDOSIS AS INDUCED BY AMPHIPHILIC CATIONIC DRUGS
FREEZE-FRACTURE STUDIES OF CYTOPLASMIC INCLUSIONS OCCURRING IN EXPERIMENTAL LIPIDOSIS AS INDUCED BY AMPHIPHILIC CATIONIC DRUGS
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DOI：
10.1016/0005-2760(79)90132-2
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
LULLMANNRAUCH, R
中科院分区：
文献类型：
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作者：
BUCHHEIM, W;DRENCKHAHN, D;LULLMANNRAUCH, R
采用冷冻破裂和薄切片的方法研究了几种两亲性阳离子药物诱导实验性脂质沉积的细胞质包涵体的超微结构。使用大鼠视网膜和肾上腺组织长期口服高剂量氯苯定。在细胞质包涵体的薄片上，先前被证明代表了具有极性脂质超载的溶酶体，显示出层状或晶格状的内部模式。冻裂观察结果表明，片状包裹体在片层相中含有极性脂质，而晶格样包裹体在六边形相中含有极性脂质。
The ultrastructure of cytoplasmic inclusions, which characterizes experimental lipidosis as induced by several amphiphilic cationic drugs, was studied by freeze-fracturing and thin-sectioning. Retinal and adrenal tissues of rats chronically treated with high oral doses of chlorphentermine were used. In thin sections the cytoplasmic inclusions, which were previously shown to represent lysosomes overloaded with polar lipids, exhibit lamellated or lattice-like internal patterns. The present freeze-fracture observations apparently indicate that the lamellated inclusions contain polar lipids in the lamellar phase, whereas those with lattice-like patterns contain polar lipids in a hexagonal phase.