Staining ability and biocompatibility of brilliant blue G - Preclinical study of brilliant blue G as an adjunct for capsular staining
Staining ability and biocompatibility of brilliant blue G - Preclinical study of brilliant blue G as an adjunct for capsular staining
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DOI：
10.1001/archopht.124.4.514
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发表时间：
2006-04-01
影响因子：
--
通讯作者：
Ishibashi, T
中科院分区：
文献类型：
--
作者：
Hisatomi, T;Enaida, H;Ishibashi, T
目的：评价亮蓝G （BBG）用于连续曲线型荚膜撕裂的荚膜显像效果及生物相容性。方法：分别以10.0、1.0、0.5、0.25、0.1、0.01 mg/mL的浓度对去核猪眼进行染色。在大鼠眼内观察BBG的生物相容性2个月。用光学、荧光、透射电子和扫描电子显微镜对眼睛进行分析。TUNEL（末端脱氧核苷酸转移酶介导的生物素脱氧尿苷三磷酸镍端标记）检测凋亡细胞，扫描电镜分析内皮细胞计数。用吲哚菁绿和台盼蓝对结果进行比较。结果：BBG改善了囊膜的可视性，可实现完全的囊膜撕裂。在大鼠模型中，2个月生物显微镜下未观察到明显的毒性作用。组织学上，BBG表现出良好的生物相容性。仅台盼蓝组可见内皮细胞凋亡。透射电镜和扫描电镜下，吲哚菁绿和台盼蓝显示角膜内皮细胞变性。结论：BBG具有较好的囊膜显像效果，对角膜内皮无明显并发症。临床意义：BBG对连续曲线型囊裂的连续曲线染色是有效和安全的。
Objective: To evaluate the effectiveness and biocompatibility of brilliant blue G (BBG) for capsular visualization for continuous curvilinear capsulorrhexis.Methods: The capsular staining ability of BBG was evaluated at graded concentrations of 10.0, 1.0, 0.5, 0.25, 0.1, and 0.01 mg/mL in enucleated pig's eyes. The biocompatibility of BBG was assessed in rat's eyes for 2 months. The eyes were analyzed using light, fluorescence, transmission electron, and scanning electron microscopy. TUNEL (terminal deoxynucleotidyl transferase-mediated biotin deoxyuridine triphosphate nick-end labeling) was used to detect apoptotic cells, and endothelial cell counts were analyzed using scanning electron microscopy. The results were compared using indocyanine green and trypan blue.Results: The BBG improved capsular visualization, and a complete capsulorrhexis could be performed. In the rat model, no apparent toxic effect was observed using biomicroscopy during 2 months. Histologically, BBG showed satisfactory biocompatibility. Apoptotic cell death of the endothelial cells was detected in only the trypan blue group. In contrast to BBG, indocyanine green and trypan blue showed degeneration of corneal endothelial cells using transmission and scanning electron microscopy.Conclusion: The BBG contributed to better capsular visualization and caused no apparent complications to the corneal endothelium.Clinical Relevance: The BBG is effective and safe capsular staining for continuous curvilinear staining for continuous curvilinear capsulorrhexis.