16, 16-DIMETHYL PROSTAGLANDIN-E2 INCREASES SURVIVAL OF MURINE INTESTINAL STEM-CELLS WHEN GIVEN BEFORE PHOTON RADIATION
16, 16-DIMETHYL PROSTAGLANDIN-E2 INCREASES SURVIVAL OF MURINE INTESTINAL STEM-CELLS WHEN GIVEN BEFORE PHOTON RADIATION
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DOI：
10.2307/3576222
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发表时间：
1983-01-01
影响因子：
3.4
通讯作者：
THOMAS, C
中科院分区：
文献类型：
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作者：
HANSON, WR;THOMAS, C
当在诸如无水乙醇、酸化牛磺胆酸盐、沸水或非甾体抗炎药（NSAI）等损伤剂之前给药时，多种甘草素（PG）可保护胃和肠粘膜。在137 Cs全身γ-干扰前1小时或137 Cs全身γ-干扰后15分钟给予小鼠一种合成的前列腺素，16，16-二甲基PGE 2，其显示出在生理水平上对上述试剂具有细胞保护作用。辐照当在137 Cs γ-之前而不是之后给予16，16-二甲基PGE 2时，通过肠干细胞形成再生上皮的原位集落的能力测量的肠干细胞的存活增加。辐照16，16-二甲基前列腺素E2诱导的辐射防护作用在照射前1h达到最大。当药物和照射之间的间隔为3小时或更长时，没有观察到辐射保护作用。当16，16-二甲基PGE_2与照射间隔时间为1h时，PG的辐射防护作用与PG的剂量有关。在低剂量的PG下存活细胞的数量急剧上升。这些结果意味着分泌PGE 2的肿瘤可能部分受到电离光子辐射致死效应的保护。
A variety of prostaglandins (PG) protect the gastric and intestinal mucosa when given before damaging agents such as absolute ethanol, acidified taurocholate, boiling water or nonsteroidal anti-inflammatory agents (NSAI). A synthetic prostaglandin, 16,16-dimethyl PGE2, shown to be cytoprotective at physiologic levels to the above agents was given to mice 1 h before or 15 min after 137Cs whole-body .gamma.-irradiation. The survival of intestinal stem cells measured by their ability to form in situ colonies of regenerating epithelium was increased when 16,16-dimethyl PGE2 was given before but not after 137Cs .gamma.-irradiation. The maximum degree of 16,16-dimethyl PGE2-induced radioprotection was seen when the drug was given 1 h before irradiation. No radioprotection was seen when the interval between drug and irradiation was 3 h or longer. When the time between 16,16-dimethyl PGE2 and irradiation was kept at 1 h, the degree of radioprotection was dependent on the PG drug dose. There was a steep rise in the number of surviving cells at low doses of PG. These results imply that tumors which secrete PGE2 may in part be protected from the lethal effects of ionizing photon radiation.