Expression of inflammatory cytokines, RANKL and OPG induced by titanium, cobalt-chromium and polyethylene particles
Expression of inflammatory cytokines, RANKL and OPG induced by titanium, cobalt-chromium and polyethylene particles
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DOI：
10.1016/j.biomaterials.2004.05.017
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Ishiguro, N
中科院分区：
文献类型：
--
作者：
Masui, T;Sakano, S;Ishiguro, N
骨吸收受肿瘤坏死因子-α (TNF-α)、白细胞介素-1 β (IL-1β) 等细胞因子以及核因子 kappaB 配体 (RANKL) 受体激活剂和骨保护素 (OPG) 平衡的调节。本研究的目的是通过评估植入金属和聚乙烯颗粒后骨溶解的程度以及炎症细胞因子、RANKL 和 OPG 的表达来研究颗粒诱导小鼠颅骨骨溶解的机制。植入 Ti6Al4V、CoCr 或高密度聚乙烯 (HDP) 颗粒的小鼠颅骨显示出更广泛的骨质溶解和炎症细胞因子水平的升高。植入 Ti6Al4V 和 HDP 颗粒的小鼠中 RANKL 和 OPG 的比率较高，但植入 CoCr 颗粒的小鼠中 RANKL 和 OPG 的比率较高。这些观察结果表明，CoCr 颗粒诱导的破骨细胞生成可能是由炎症细胞因子直接引起的，而不是由 RANK-RANK 途径引起的。 Ti6Al4V 之间颗粒诱导的骨溶解可能存在不同的机制。 HDP 和钴铬。 (C) 2004 Elsevier Ltd. 保留所有权利。
Bone resorption is regulated by cytokines such as tumor necrosis factor-alpha (TNF-alpha), intereukin-1 beta (IL-1beta) and by the balance of a receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoprotegerin (OPG). The aim of this study was to investigate the mechanism of particle-induced osteolysis in murine calvariae by assessing the extent of osteolysis and the expression of inflammatory cytokines, RANKL and OPG after implantation of metal and polyethylene particles. The murine calvariae implanted with Ti6Al4V, CoCr or high-density polyethylene (HDP) particles showed significantly more extensive osteolysis and elevated levels of inflammatory cytokines. The ratio between RANKL and OPG was high in the mice implanted with Ti6Al4V and HDP particles, but not in the mice implanted with CoCr particles. These observations suggested that CoCr particle-induced osteoclastogenesis may be caused directly by inflammatory cytokines rather than by the RANK-RANK pathway. There might be different mechanisms at work in particle-induced osteolysis between Ti6Al4V. HDP and CoCr. (C) 2004 Elsevier Ltd. All rights reserved.