A specific set of EJC subunits is required for the nuclear retention of unspliced RNAs in C. elegans
A specific set of EJC subunits is required for the nuclear retention of unspliced RNAs in C. elegans
复制标题
秀丽隐杆线虫中未剪接 RNA 的核保留需要一组特定的 EJC 亚基
DOI：
10.1128/mcb.01298-12
复制
发表时间：
2013
影响因子：
5.3
通讯作者：
Hiroshi Sakamoto
中科院分区：
文献类型：
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作者：
Masami Shiimori;KunioInoue;Hiroshi Sakamoto
外显子连接复合体（exon junction complex，EJC）在许多生物体中高度保守，并且参与mRNA代谢的各个步骤。在研究EJC在秀丽隐杆线虫生殖系性别决定中的作用过程中，我们发现EJC的三个核心亚基（Y14、MAG-1和真核翻译起始因子4 III [eIF 4AIII]）之一或一个辅助亚基（UAP 56）的缺失导致几乎所有线虫未剪接RNA的胞质泄漏。elegansprotein编码基因检测到目前为止。这种泄漏也观察到了几种剪接因子的耗尽，包括SF 3b，IBP 160和PRP 19，所有这些都与Y14发生遗传相互作用。我们还发现，Y14与前mRNA和剪接体U snRNA，特别是U2 snRNA的物理相互作用，并且当IBP 160和PRP 19都耗尽时，相互作用被消除。我们的研究结果强烈表明，一组特定的EJC亚基被募集到内含子上，并与剪接体的组分，包括U2 snRNP相互作用，为C中未剪接RNA的监视和核保留提供关键信号。优美的
The exon junction complex (EJC) is highly conserved in many organisms and is involved in various steps of mRNA metabolism. During the course of investigating the role of EJC in the germ line sex determination of the nematodeCaenorhabditis elegans, we found that depletion of one of the three core subunits (Y14, MAG-1, and eukaryotic translation initiation factor 4III [eIF4AIII]) or one auxiliary subunit (UAP56) of EJC resulted in the cytoplasmic leakage of unspliced RNAs from almost all of theC. elegansprotein-coding genes examined thus far. This leakage was also observed with the depletion of several splicing factors, including SF3b, IBP160, and PRP19, all of which genetically interacted with Y14. We also found that Y14 physically interacts with both pre-mRNA and spliceosomal U snRNAs, especially U2 snRNA, and that the interaction was abolished when both IBP160 and PRP19 were depleted. Our results strongly suggest that a specific set of EJC subunits is recruited onto introns and interacts with components of the spliceosome, including U2 snRNP, to provide a critical signal for the surveillance and nuclear retention of unspliced RNAs inC. elegans.