Mineral trioxide aggregate promotes the odonto/osteogenic differentiation and dentinogenesis of stem cells from apical papilla via nuclear factor kappa B signaling pathway.
Mineral trioxide aggregate promotes the odonto/osteogenic differentiation and dentinogenesis of stem cells from apical papilla via nuclear factor kappa B signaling pathway.
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DOI：
10.1016/j.joen.2014.01.042
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发表时间：
2014-05
影响因子：
4.2
通讯作者：
Ming Yan;Jintao Wu;Yan Yu;Yanping Wang;Lizhe Xie;Guangdong Zhang;Jinhua Yu;Chengfei Zhang
中科院分区：
文献类型：
--
作者：
Ming Yan;Jintao Wu;Yan Yu;Yanping Wang;Lizhe Xie;Guangdong Zhang;Jinhua Yu;Chengfei Zhang
三氧化矿物聚合体(MTA)已广泛应用于临床根尖诱导成形术。然而，MTA对尖端乳头干细胞(SCAPs)的作用及其确切的发生机制尚不清楚。方法从根尖乳头分离出多个集落来源的干细胞，并在体内观察MTA对SCAPs增殖和分化的影响。用免疫荧光法和免疫印迹法检测核转录因子-kappaB(NF-κ-B)通路的激活。结果2 mg/mLMTA对SCAP的增殖活性无明显影响。2 mg/mLMTA作用后SCAPs的超微结构发生改变，碱性磷酸酶上调，钙沉积增加，成牙本质细胞标志物(牙本质唾液蛋白和牙本质涎磷蛋白)和成牙本质/成骨细胞标志物(矮小相关转录因子2和骨钙素)表达上调，提示MTA促进SCAPsin体外成牙/成骨分化。此外，MTA处理的SCAP细胞胞浆内PHOS-IκBα和PHOS-p65表达上调，核内p65表达增强，p65核转位增加。结论2 mg/mLMTA可通过激活NFκB途径提高SCAPs的成牙本质/成骨能力。
IntroductionMineral trioxide aggregate (MTA) has been widely used in clinical apexification and apexogenesis. However, the effects of MTA on the stem cells from apical papilla (SCAPs) and the precise mechanism of apexogenesis have not been elucidated in detail.MethodsMultiple colony-derived stem cells were isolated from the apical papillae, and the effects of MTA on the proliferation and differentiation of SCAPs were investigated bothin vitroandin vivo. Activation of nuclear factor kappa B (NFκB) pathway in MTA-treated SCAPs was analyzed by immunofluorescence assay and Western blot.ResultsMTA at the concentration of 2 mg/mL did not affect the proliferation activity of SCAPs. However, 2 mg/mL MTA-treated SCAPs presented the ultrastructural changes, up-regulated alkaline phosphatase, increased calcium deposition, up-regulated expression of odontoblast markers (dentin sialoprotein and dentin sialophosphoprotein) and odonto/osteoblast markers (runt-related transcription factor 2 and osteocalcin), suggesting that MTA enhanced the odonto/osteoblastic differentiation of SCAPsin vitro.In vivoresults confirmed that MTA can promote the regular dentinogenesis of SCAPs. Moreover, MTA-treated SCAPs exhibited the up-regulated cytoplasmic phos-IκBα and phos-P65, enhanced nuclear P65, and increased nuclear translocation of P65. When co-treated with BMS345541 (the specific NFκB inhibitor), MTA-mediated odonto/osteoblastic differentiation was significantly attenuated.ConclusionsMTA at the concentration of 2 mg/mL can improve the odonto/osteogenic capacity of SCAPs via the activation of NFκB pathway.