Determination of Uric Acid using 4-Amino-3-hydrazino-5-mercapto-1,2,4-triazole and Urate Oxidase-Catalase System
Determination of Uric Acid using 4-Amino-3-hydrazino-5-mercapto-1,2,4-triazole and Urate Oxidase-Catalase System
复制标题
使用 4-氨基-3-肼基-5-巯基-1,2,4-三唑和尿酸氧化酶-过氧化氢酶系统测定尿酸
DOI：
10.1248/cpb.26.1945
复制
发表时间：
1978
影响因子：
1.7
通讯作者：
T. Suga
中科院分区：
文献类型：
--
作者：
Takafumi Watanabe;T. Suga
A new method of colorimetric determination of uric acid, using uricase and catalase is reported. This method is based on the combination of enzyme reactions and colorimetric procedure with using 4-amino-3-hydrazino-1, 2, 4-triazole, a highly sensitive reagent for formaldehyde. The color developed in a straight line passing through the original point at least within the absorbance unit of 1.5 at 550 nm, corresponding to 16 mg/dl of the uric acid concentration of the sample.
