Electrochemiluminescence at Bare and DNA-Coated Graphite Electrodes in 3D-Printed Fluidic Devices.
Electrochemiluminescence at Bare and DNA-Coated Graphite Electrodes in 3D-Printed Fluidic Devices.
复制标题
3D打印的流体设备中的裸露和DNA涂层的石墨电极处的电化学发光。
DOI：
10.1021/acssensors.5b00156
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发表时间：
2016
期刊：
影响因子：
8.9
通讯作者：
Rusling JF
中科院分区：
文献类型：
--
作者：
Bishop GW;Satterwhite-Warden JE;Bist I;Chen E;Rusling JF
使用基于立体光刻的低成本桌面三维（3D）打印机制备具有用于并入电极以实现电化学发光（ECL）测量的端口的透明塑料流体装置。电极由0.5 mm铅笔状石墨棒和0.5 mm银线组成，插入市售1/4 in. 28个螺纹接头。使用配备有CCD相机的生物成像系统测量在电极和小阵列处产生的ECL，使用含有三（2，2 ′-联吡啶）二氯钌（II）六水合物（[Ru（bpy）3] 2+）和100 mM三正丙胺（TPA）作为共反应物的0.2 M磷酸盐缓冲溶液。当[Ru（bpy）3] 2+浓度为9-900 μ M时，在铅笔石墨工作电极上产生的ECL信号与[Ru（bpy）3] 2+浓度呈线性关系。使用曝光时间设定为10 s的CCD相机，发现检测限为7 μ M。电极间ECL信号变化± 7.5%。使用涂覆有多层聚（二烯丙基二甲基氯化铵）（PDDA）/DNA膜的铅笔石墨电极进一步评价装置性能。在这些实验中，[Ru（bpy）3] 3+与DNA的鸟嘌呤反应产生电致发光。在180~800 μ M范围内，[Ru（bpy）3] 2+浓度与电化学发光呈线性关系。这些研究首次证明了使用3D打印的封闭通道流体装置平台获得的ECL测量结果。在这些研究中使用的经济实惠的高分辨率3D打印机可以实现简单，快速和适应性强的流体设备原型设计，这些设备能够包含用于测量ECL的电极。
Clear plastic fluidic devices with ports for incorporating electrodes to enable electrochemiluminescence (ECL) measurements were prepared using a low-cost, desktop three-dimensional (3D) printer based on stereolithography. Electrodes consisted of 0.5 mm pencil graphite rods and 0.5 mm silver wires inserted into commercially available 1/4 in.-28 threaded fittings. A bioimaging system equipped with a CCD camera was used to measure ECL generated at electrodes and small arrays using 0.2 M phosphate buffer solutions containing tris(2,2′-bipyridyl)dichlororuthenium(II) hexahydrate ([Ru(bpy)3]2+) with 100 mM tri-n-propylamine (TPA) as the coreactant. ECL signals produced at pencil graphite working electrodes were linear with respect to [Ru(bpy)3]2+ concentration for 9–900 μM [Ru(bpy)3]2+. The detection limit was found to be 7 μM using the CCD camera with exposure time set at 10 s. Electrode-to-electrode ECL signals varied by ±7.5%. Device performance was further evaluated using pencil graphite electrodes coated with multilayer poly(diallyldimethylammonium chloride) (PDDA)/DNA films. In these experiments, ECL resulted from the reaction of [Ru(bpy)3]3+ with guanines of DNA. ECL produced at these thin-film electrodes was linear with respect to [Ru(bpy)3]2+ concentration from 180 to 800 μM. These studies provide the first demonstration of ECL measurements obtained using a 3D-printed closed-channel fluidic device platform. The affordable, high-resolution 3D printer used in these studies enables easy, fast, and adaptable prototyping of fluidic devices capable of incorporating electrodes for measuring ECL.