DEVELOPMENT AND TESTING OF THE AIN-93 PURIFIED DIETS FOR RODENTS - RESULTS ON GROWTH, KIDNEY CALCIFICATION AND BONE MINERALIZATION IN RATS AND MICE
DEVELOPMENT AND TESTING OF THE AIN-93 PURIFIED DIETS FOR RODENTS - RESULTS ON GROWTH, KIDNEY CALCIFICATION AND BONE MINERALIZATION IN RATS AND MICE
复制标题
DOI：
10.1093/jn/123.11.1923
复制
发表时间：
1993-11-01
影响因子：
4.2
通讯作者：
LINDLAUF, J
中科院分区：
文献类型：
--
作者：
REEVES, PG;ROSSOW, KL;LINDLAUF, J
由于营养和技术问题，AIN-76A 啮齿动物饮食进行了修改。其中一种新配方被命名为 AIN-93G，建议用于生长、妊娠和哺乳研究。与AIN-76A日粮相比，这种新配方的一些主要差异如下：用7克大豆油代替5克玉米油/100克日粮，以增加亚麻酸的含量；维生素E和K的含量增加；玉米淀粉代替了大部分蔗糖；减少磷用量，消除雌性大鼠肾钙质沉着问题； L-胱氨酸替代DL-蛋氨酸；且锰浓度降低。将 AIN-93G 饮食的各种发育改良喂养给断奶大鼠和小鼠，以确定对生长和组织矿化的影响。给大鼠喂食发育版 AIN-93G 16 周后，雄性和雌性大鼠的体重与喂食以谷物为基础的非纯化饮食的大鼠没有差异。 13周后，喂食这种饮食的雄性小鼠比喂食非纯化饮食的雄性小鼠体重增加了13%。雌性小鼠的体重没有受到影响。新的饮食配方在 16 周的喂养期间可防止雌性大鼠和小鼠的肾脏钙化。
Because of nutritional and technical problems the AIN-76A rodent diet was revised. One of the new formulations was designated AIN-93G and was suggested for use during growth, pregnancy and lactation studies. Some major differences in this new formulation compared with the AIN-76A diet are as follows: 7 g soybean oil was substituted for 5 g corn oil/100 g diet to increase the amount of linolenic acid; the amounts of vitamins E and K were increased; cornstarch was substituted for most of the sucrose; the amount of phosphorus was reduced to eliminate the problem of nephrocalcinosis in female rats; L-cystine was substituted for DL-methionine; and the manganese concentration was reduced. Various developmental modifications of the AIN-93G diet were fed to weanling rats and mice to determine effects on growth and tissue mineralization. After rats were fed the developmental version of AIN-93G for 16 wk, body weights in both male and female rats were not different from those of rats fed a cereal-based nonpurified diet. After 13 wk, male mice fed this diet weighed 13% more than those fed the nonpurified diet. Body weights of female mice were not affected. The new diet formulation prevented kidney calcification in female rats and mice during 16 wk of feeding.