Microelectrode array for chronic deep-brain micro stimulation and recording
Microelectrode array for chronic deep-brain micro stimulation and recording
复制标题
DOI：
10.1109/tbme.2006.870215
复制
发表时间：
2006-04-01
影响因子：
4.6
通讯作者：
Liu, XD
中科院分区：
文献类型：
--
作者：
McCreery, D;Lossinsky, A;Liu, XD
我们已经开发出一种微电极阵列，适合长期植入丘脑底核（subthalamic nucleus，简称ENA）或苍白球，能够记录单个神经元，并提供局部微刺激。该装置可用于研究脑深部电刺激改善帕金森病和其他运动障碍症状的机制，也可能是利用核内微刺激的高度空间特异性治疗帕金森病的新临床工具的基础。该阵列包括16个活化的铱微电极，长度为5-6 mm，在直径约1.8 mm的簇内。我们已经制作了阵列使用的材料进行USP第六类分类，我们已经开发了一种装置和程序植入到脑深部的微电极阵列。将10个阵列植入家猫的直肠，一个植入苍白球的内部，持续140-415天。在此期间，我们能够记录单个神经元的动作电位，在16个通道中的4到8个通道上。微电极的活性表面积范围为500至2000 μ m（2）。控制电流脉冲，26.5 μ A的幅度和150 μ s/相的持续时间（4 nC/相）被用来激发猫的脊髓神经元。除了直接激活，刺激调制的神经元活动超过至少1.2毫米的距离从刺激的网站。如果电极的有效表面积为1000 μ m（2）或更大，则在100 Hz刺激35小时（每天刺激7小时，连续5天）后，这些参数不会引起尖端部位周围的组织学可检测变化。
We have developed an array of microelectrodes that is suitable for long-term implantation into the subthalamic nucleus (STN) or the globus pallidus and is able to record from single neurons, as well as deliver localized microstimulation. This device can be used to investigate the mechanisms by which deep brain stimulation can ameliorate the symptoms of Parkinson's disease and other movement disorders, and also may be the basis for a new clinical tool for the treatment of Parkinson's disease, by capitalizing on the high spatial specificity of intranuclear microstimulation. The array includes 16 activated iridium microelectrodes, 5-6 mm in length, within a cluster approximately 1.8 mm in diameter. We have fabricated the array using materials carrying the USP Category VI classification, and we have developed an apparatus and a procedure for implanting the microelectrode arrays into the deep brain. Ten arrays have been implanted into the STN of domestic cats, and one into the internal segment of the globus pallidus, for 140-415 days. During that time, we were able to record action potentials from individual neurons, on 4 to 8 of the 16 channels. The microelectrode' active surface areas ranged from 500 to 2000 mu m(2). Controlled-current pulses, 26.5 mu A in amplitude and 150 mu s/phase in duration (4 nC/phase) were used to excite neurons in the cat's STN. In addition to direct activation, the stimulus modulated the neuronal activity over a distance of at least 1.2 mm from the site of stimulation. These parameters did not induce histologically detectable changes around the tip sites after 35 hours of stimulation at 100 Hz (7 hours of stimulation per day, on 5 successive days), if the electrode' active surface area was 1000 mu m(2) or greater.