Phylogenetic analysis of the 16S rDNA of the cytoplasmic bacterium Wolbachia from the novel host Folsomia candida (Hexapoda, Collembola) and its implications for wolbachial taxonomy
Phylogenetic analysis of the 16S rDNA of the cytoplasmic bacterium Wolbachia from the novel host Folsomia candida (Hexapoda, Collembola) and its implications for wolbachial taxonomy
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DOI：
10.1111/j.1574-6968.1999.tb08807.x
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发表时间：
1999-11-15
影响因子：
2.1
通讯作者：
Gillis, M
中科院分区：
文献类型：
--
作者：
Vandekerckhove, TTM;Watteyne, S;Gillis, M
细管沃尔巴克氏菌是无脊椎动物细胞内、跨脊椎动物遗传的α-变形杆菌。有四个主要的沃尔巴克氏菌群：A，B(包含在不同的节肢动物中)，C和D(由线虫组成)。利用透射电子显微镜观察了原始昆虫--拟沃尔巴克氏菌，16S rDNA分析证实它们构成了沃尔巴克氏系统发育树上的一个新的谱系，此后命名为E群。它与其最近的邻居A和B组有97.8%的16S rDNA同源性，后者最近与它发生了分歧。我们建议(I)在假丝酵母中为Wolbachia菌株建立一个新的分类群E，(Ii)单个描述的Wolbachia pipientis至少分为三个种：C，D和大的E-A-B复合体。念珠菌的E群沃尔巴克氏菌重新点燃了关于自由生活的祖先沃尔巴克氏菌的入侵能力和水平转移的问题。(C)1999年欧洲微生物学会联合会。爱思唯尔科学公司出版。版权所有。
Wolbachia pipientis are intracellular, transovarially inherited alpha-Proteobacteria in invertebrates. Four major Wolbachia groups exist: A, B (contained in divergent arthropods), C and D (harbored by Nematoda). By means of transmission electron microscopy, we observed Wolbachia-like bacteria in a primitive insect, Folsomia candida (Hexapoda, Collembola, Isotomidae), 16S rDNA analysis proved them to constitute a novel lineage, henceforth named group E, in the wolbachial phylogenetic tree. It shares 97.8% 16S rDNA homology with its nearest neighbors, groups A and B, which diverged from it more recently. We propose (i) a new taxon E for the Wolbachia strain in F. candida, (ii) that the single-described Wolbachia pipientis fall apart into at least three species: C, D and the large E-A-B complex. F. candida's group E Wolbachia rekindle the question about invasive capacities of free-living ancestral wolbachiae and horizontal transfer. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.