Genome‐wide organization, evolutionary diversification of the COMMD family genes of amphioxus (Branchiostoma belcheri) with the possible role in innate immunity
Genome‐wide organization, evolutionary diversification of the COMMD family genes of amphioxus (Branchiostoma belcheri) with the possible role in innate immunity
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DOI：
10.1016/j.fsi.2018.03.019
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发表时间：
2018-06
影响因子：
4.7
通讯作者：
P. Jin;Caiyun Lv;Shuangli Peng;Lu Cai;Jiu Zhu;Fei Ma
中科院分区：
文献类型：
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作者：
P. Jin;Caiyun Lv;Shuangli Peng;Lu Cai;Jiu Zhu;Fei Ma
铜代谢基因MURR1域基因家族由10个成员组成，参与铜、钠转运、核因子-κB活性调节和细胞周期进程等多种生物学过程。然而，关于文昌鱼(Branchiostoma Belcheri)中COMMD基因家族的研究还很少。在本研究中，我们从文昌鱼中鉴定并鉴定了tenCOMMD家族成员(命名为AmphiCOMMDs)。首先，我们克隆了AmphiCOMMD的全长，所有的AmphiCOMMD蛋白都含有保守的COMM结构域和两个核输出信号(NES)基序。其次，基因组结构分析表明，在文昌鱼向脊椎动物分化的过程中，COMM家族的基因经历了内含子的丢失和获得。系统发育分析表明，AmphiCOMMDs与脊椎动物的亲缘关系较近，可能是脊椎动物COMMDs的祖先。第四，AmphiCOMM在所研究的五种组织(肌肉、鳃、肠、肝性盲肠和脊索)中普遍存在并存在差异表达。最后，我们的结果表明，在内毒素刺激后，AmphiCOMMD基因的表达水平出现了不同程度的波动。综上所述，我们的研究阐述了文昌鱼COMM家族基因的进化动态和先天免疫作用。
TheCOMMD(COpper Metabolism gene MURR1 Domain) gene family with ten members participates in various biological processes, such as the regulation of copper and sodium transport, NF-κB activity and cell cycle progression. However, studies on theCOMMDgene family in amphioxus (Branchiostoma belcheri) are yet largely unknown. In this study, we have identified and characterized the tenCOMMDfamily members from amphioxus (designated asAmphiCOMMDs). Firstly, we clone the full length ofAmphiCOMMDs, and all AmphiCOMMD proteins contain the conserved COMM domain with two NES (Nuclear Export Signal) motifs. Secondly, the genomic structure analysis demonstrates that genes of theCOMMDfamily have undergone intron loss and gain during the process of divergence from amphioxus to vertebrates. Thirdly, phylogenetic analysis indicates thatAmphiCOMMDs are more closely related to vertebrates, implying theAmphiCOMMDsmay be the ancestor of the vertebrateCOMMDs. Fourthly,AmphiCOMMDsare ubiquitously and differentially expressed in five investigated tissues (muscles, gills, intestine, heaptic cecum and notochord). Finally, our results show that expression levels ofAmphiCOMMDgenes are fluctuating after LPS stimulation to some different extent. Taken together, our studies have elaborated the evolutionary dynamic and the innate immune role of theCOMMDfamily genes in amphioxus.