A thermodynamic study on the binding of magnesium with human growth hormone - Consideration of the new extended coordination model solvation parameters
A thermodynamic study on the binding of magnesium with human growth hormone - Consideration of the new extended coordination model solvation parameters
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DOI：
10.1007/s10973-006-8206-x
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发表时间：
2007-09-01
影响因子：
4.4
通讯作者：
Saboury, A. A.
中科院分区：
文献类型：
--
作者：
Behbehani, G. Rezaei;Saboury, A. A.
利用等温滴定量热法测定Mg 2+与人生长激素hGH疏水核心结合的热力学参数。在27 ℃ NaCl溶液中研究了Mg 2+和hGH（35 μ M）之间的相互作用。使用新的溶剂化模型重现整个Mg 2+浓度下Mg 2 +-hGH相互作用的曲线。从新的溶剂化模型恢复的溶剂化参数，与由于金属离子相互作用的hGH的结构变化。
The thermodynamic parameters underlying the binding of Mg2+ to the hydrophobic core of human growth hormone, hGH, are determined using isothermal titration calorimetry. The interaction between Mg2+ and hGH (35 mu M) was studied at 27 degrees C in NaCl solution. A new solvation model was used to reproduce the enthalpies of Mg2+-hGH interaction over the whole Mg2+ concentrations. The solvation parameters recovered from the new salvation model, were correlated to the structural changes of hGH due to the metal ion interaction.