Detection of Cytomegalovirus (CMV) Infection in Wheezing Infants by Urine DNA and Serum IgG Testing.
Detection of Cytomegalovirus (CMV) Infection in Wheezing Infants by Urine DNA and Serum IgG Testing.
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DOI：
10.12659/msm.898589
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发表时间：
2017-03-11
期刊：
影响因子：
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通讯作者：
Yang X
中科院分区：
文献类型：
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作者：
Zeng ZC;Chang Q;Sun ZW;Song MM;Jin XL;Jiang SY;Yang X
本研究的目的是探讨巨细胞病毒感染与喘息婴儿的关系，以及巨细胞病毒DNA与免疫球蛋白之间的关系。共有243名喘息的婴儿和3000名产妇参加了这项研究。婴儿随机分组，分别接受血和尿中HCMV-DNA检测(n=46和n=197)。此外，所有参与者都进行了血清CMV特异性IgM和IgG检测。随机抽取10例HCMV-Ig G阳性婴儿同时进行血、尿HCMV-DNA检测，随机抽取25例HCMV-Ig G阳性产妇进行尿液HCMV-DNA检测。尿液中人巨细胞病毒脱氧核糖核酸的检出率显著高于血中巨细胞病毒脱氧核糖核酸(67.5%对13.0%，p<；0.001)。15名婴儿(6.2%)和190名婴儿(80.0%)巨细胞病毒特异性免疫球蛋白M和免疫球蛋白结果阳性(p<；0.001)。在进一步检测的10例HCMV-Ig G阳性婴儿中，仅2例血HCMV-DNA阳性，10例尿液中均为阳性。而随机抽取的25例HCMV-Ig G阳性产妇尿液中未检出HCMV-DNA。CMV感染可能是婴幼儿喘息的原因之一，尿HCMV-DNA和血清HCMV-Ig G检测可检测到CMV感染。婴儿比产妇更容易感染巨细胞病毒。
The aim of this study was to investigate the involvement of CMV infection in wheezing infants and the association between CMV-DNA and immunoglobulins (Igs). A total of 243 wheezing infants and 3,000 parturients were enrolled in this study. The infants were randomly grouped to receive blood HCMV-DNA tests (n=46) or urine HCMV-DNA tests (n=197). Furthermore, all participants had serum CMV-specific IgM and IgG testing. Afterwards, 10 HCMV-IgG positive infants were randomly selected for simultaneous blood and urine HCMV-DNA tests, and 25 HCMV-IgG positive puerperants were randomly selected for urine HCMV-DNA tests. The detection rate of urine HCMV-DNA was significantly higher than that of blood HCMV-DNA (67.5% vs. 13.0%, p<0.001). Fifteen (6.2%) and 190 (80.0%) infants showed positive CMV-specific IgM and IgG results (p<0.001), respectively. Among the 10 HCMV-IgG positive infants tested further, only two infants had positive HCMV-DNA blood tests, while all of the 10 infants had positive HCMV-DNA urine tests. However, HCMV-DNA was not detected in the urine of the 25 randomly selected parturients positive for HCMV-IgG. CMV infection may be one of the causes of wheezing in infants; CMV infection can be detected by urine-HCMV-DNA and serum HCMV-IgG testing. Infants were more susceptible to CMV infection than parturients.