Cromolyn inhibition of mediator release in mast cells derived from mouse bone marrow.
Cromolyn inhibition of mediator release in mast cells derived from mouse bone marrow.
复制标题
色甘酸抑制小鼠骨髓肥大细胞中介质的释放。
DOI：
10.1164/arrd.1986.133.6.1105
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Wasserman,SI
中科院分区：
文献类型：
--
作者：
Marquardt,DL;Walker,LL;Wasserman,SI
来自小鼠骨髓的肥大细胞未能表现出二钠色甘酸诱导的抑制介质释放时，cron同时加入促分泌素。为了研究克罗维因暴露的长期影响，在1 - 100 μM克罗维因存在下培养经IL-3刺激的BALB/C股骨骨髓细胞，并显示与单独培养在培养基中的细胞相似的活力和增殖能力。与对照细胞相比，在croplyn中培养的那些细胞表现出显著降低的A23187或抗原诱导的β-氨基己糖苷酶释放（4.3 ± 0.7%对9.2 ± 1.0%，p < 0.01）。腺苷诱导的增强介质的释放减少到一个相当的程度后，crologyn暴露在文化中。这种抑制作用在1 - 100 μM克罗维因之间呈剂量依赖性，5天后明显，6天后可逆，也适用于免疫反应性白三烯C4的生成。骨髓肥大细胞对慢性克罗埃西亚暴露的反应是对介质释放的全面抑制，但这种反应的基础尚不清楚。
Mast cells derived from mouse bone marrow fail to demonstrate a disodium-cromoglycate-induced inhibition of mediator release when the cromolyn is added simultaneously with a secretagogue. To investigate the long-term effects of cromolyn exposure, cells from BALB/C femoral bone marrow stimulated with IL-3 were cultured in the presence of 1 to 100 μM cromolyn and demonstrated a similar viability and ability to proliferate as cells cultured in medium alone. Those cultured in cromolyn exhibited a significantly decreased A23187- or antigen-induced release of β-hexosaminidase(4.3 ± 0.7% versus 9.2 ± 1.0%, p < 0.01) compared with control cells. The adenosine-induced potentiation of mediator release was diminished to a comparable degree after cromolyn exposure in culture. This inhibition was dose-dependent between 1 and 100 μM cromolyn, evident by 5 days, reversible in 6 more days, and applied to immunoreactive leukotriene C4generation as well. Bone marrow mast cells respond to chronic cromolyn exposure with a global inhibition of mediator release, but the basis for this response is unknown.