A novel approach to assessing eye irritation potential using the bovine corneal opacity and permeability assay
A novel approach to assessing eye irritation potential using the bovine corneal opacity and permeability assay
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DOI：
10.3109/15569529609048870
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发表时间：
1996-01-01
期刊：
影响因子：
--
通讯作者：
Klein, BD
中科院分区：
文献类型：
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作者：
Casterton, PL;Potts, LF;Klein, BD
最初由Gautheron描述的牛角膜混浊和渗透性测定（BCOP）现在被几个实验室用于评估单个化学品，化学品混合物和消费者配方的眼睛刺激潜力。我们已经将gatheron建立的原理应用于我们实验室中可用的设备和数据，以设计这种测定的变化。这种变化在三个方面有所不同：我们如何评估角膜不透明度，我们如何应用不透明度和渗透性测量来预测刺激潜力，以及我们如何评估固体材料。为了测量不透明度，使用紫外-可见分光光度计来测量光吸光度。这与Gautheron使用不透明度计来测量电压变化作为不透明度指标形成对比。渗透率以同样的方式测量，但我们通过将其应用于与刺激电位程度相关的结果表来解释结果的不同。角膜保持装置也已被修改，以允许直接应用固体，如洗衣粉和洗碗粉，到角膜表面。为了评估这些变化，我们分析了化妆品、盥洗用品和香水协会（CTFA）的第三阶段测试材料、欧洲外毒和化学品毒理学中心（ECETOC）眼睛刺激化学品参考库中列出的单个化学品、表面活性剂原料和安利产品的历史兔子眼睛刺激数据。高体内/体外相关性证明了我们所采用的变化与早期工作一样具有预测性，并证明了BCOP在评估眼睛刺激潜力方面具有广泛的适应性。
The bovine corneal opacity and permeability assay (BCOP) as originally described by Gautheron is now utilized by several laboratories to assess the eye irritation potential of individual chemicals, chemical blends, and consumer formulations. We have applied the principles established by Gautheron to the equipment and data available in our laboratory to devise a variation of this assay. This variation differs in three ways: how we evaluate corneal opacity, how we apply opacity and permeability measurements to predict irritation potential, and how we evaluate solid materials. For opacity measurement, a UV-VIS spectrophotometer is used to measure light absorbance. This contrasts with Gautheron's use of an opacitometer to measure changes in voltage as an indicator of opacity. Permeability is measured in the same manner but we interpret the results differently by applying them to a table of results relating to degrees of irritation potential. The corneal holding apparatus has also been modified to allow direct application of solids, such as laundry and dishwashing powders, onto corneal surface. To evaluate these changes, we assayed the Cosmetic, Toiletry and Fragrance Association's (CTFA) phase III test materials, individual chemicals listed in the European Centre for Ectotoxicity and Toxicology of Chemicals' (ECETOC) Eye Irritation Chemicals Reference Bank, surfactant raw materials, and Amway products with historical rabbit eye irritation data. High in vivo/in vitro correlations demonstrate the variations we have employed to be as predictive as earlier work and demonstrate the wide adaptability the BCOP provides to assess eye irritation potential.