TRANSPLANTATION OF FROZEN - THAWED MOUSE PRIMORDIAL FOLLICLES
TRANSPLANTATION OF FROZEN - THAWED MOUSE PRIMORDIAL FOLLICLES
复制标题
DOI：
10.1093/oxfordjournals.humrep.a138221
复制
发表时间：
1993-08-01
影响因子：
6.1
通讯作者：
GOSDEN, RG
中科院分区：
文献类型：
--
作者：
CARROLL, J;GOSDEN, RG
从幼年小鼠卵巢中分离原始卵泡，以二甲基亚砜为冷冻保护剂进行缓慢冷冻保存。解冻后，约80%的卵母细胞和65%的体细胞不含台盼蓝染料，表明细胞膜仍然完整。冷冻解冻的细胞悬浮在浆点中，然后移植到通过卵巢切除术绝育的宿主动物的卵巢囊中。冻融细胞的移植物重组成形态可区分的卵巢，产生雌激素活性的迹象。自然交配后，寄主雌性产生了正常的后代，遗传标记证明这些后代来自移植的冻融原始卵泡。
Primordial follicles were isolated from juvenile mouse ovaries and cryopreserved by slow freezing with dimethylsulphoxide as the cryoprotectant. After thawing, approximately 80% of the oocytes and 65% of the somatic cells excluded Trypan Blue dye, indicating that cell membranes were still intact. Frozen-thawed cells were suspended in plasma dots and transplanted to the ovarian bursas of host animals that had been sterilized by oophorectomy. The grafts of frozen-thawed cells reorganized into morphologically distinguishable ovaries which produced signs of oestrogenic activity. After natural mating, host females produced normal offspring that were demonstrated by genetic markers to be derived from the transplanted frozen-thawed primordial follicles.