Deposition of glomalin-related soil protein and sequestered toxic metals into watersheds.
Deposition of glomalin-related soil protein and sequestered toxic metals into watersheds.
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DOI：
10.1021/es0628598
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发表时间：
2007-04
影响因子：
11.4
通讯作者：
E. Chern;D. W. Tsai;O. Ogunseitan
中科院分区：
文献类型：
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作者：
E. Chern;D. W. Tsai;O. Ogunseitan
我们假设，球囊霉素相关的土壤蛋白（GRSP）被侵蚀到足够的数量，影响金属负荷到流域。我们测试了从城市/沿海流域沿着七个地点的堤坝土壤中提取的蛋白质中GRSP、球囊菌门真菌和金属（镉、铁、铅和锰）之间的相关性。免疫反应性（IRSP）和易提取（EE-IRSP）的球囊霉素范围分别为0.007至2.9 mg g（-1）和0.006至0.63 mg g（-1）的土壤。球囊霉素结合金属（微克mg（-1）蛋白）为Cd = 0.00-0.338; Fe = 0.5-227.7; Pb = 0.11-188.95; Mn = 2.23-784.42）。用PCR方法检测土壤中18 S和28 S核糖体DNA，在24%的样品中检测到球囊霉门真菌。G.两个地点的根内土壤含量分别为3.08 × 10（7）和1.96 × 10（3）拷贝克。估计每年流入流域的球囊霉素的IRSP为5.48 x 10（2）至7.22 x 10（4）kg，EE-IRSP为2.57 x 102至2.86 x 10（4）kg;包括2.7 kg（Cd）、6.3 x 10（3）kg（Fe）、5.06 x 10（2）kg（Pb）和1.80 x 10（3）kg（Mn）。这些数据提供了一个无处不在的土壤蛋白质及其金属含量的来源和生态命运的见解。
We hypothesized that glomalin-related soil proteins (GRSP) are eroded in sufficient quantities to influence metal loading into watersheds. We tested correlations among GRSP, Glomeromycota fungi, and metals (cadmium, iron, lead, and manganese) in proteins extracted from embankment soils at seven locations along an urban/coastal watershed. Immunoreactive (IRSP) and easily extractable (EE-IRSP) glomalin ranged from 0.007 to 2.9 mg g(-1) and from 0.006 to 0.63 mg g(-1) of soils, respectively. Glomalin-bound metals (microg mg(-1) protein) were Cd = 0.00-0.338; Fe = 0.5-227.7; Pb = 0.11-188.95; Mn = 2.23-784.42). Glomeromycota fungi were detected in 24% of all samples tested with PCR targeting the 18S and 28S ribosomal DNA extracted from soils. Specific assay for G. intraradices showed 3.08 x 10(7) copies g(-1) and 1.96 x 10(3) copies g(-1) of soil at two sites. Estimated annual glomalin loading into the watershed ranged from 5.48 x 10(2) to 7.22 x 10(4) kg of IRSP and from 2.57 x 102 to 2.86 x 10(4) kg of EE-IRSP; including 2.7 kg (Cd), 6.3 x 10(3) kg (Fe), 5.06 x 10(2) kg (Pb), and 1.80 x 10(3) kg (Mn). These data provide insights into the sources and ecological fate of a ubiquitous soil protein and its metals content.